Abstract
Besides amino acid composition of a protein, their bioavailability is an important determinant of the protein quality. In view of the observations over the last decade or two, implicating the small peptide uptake by the mammalian intestine as a major route of protein absorption, a few animal and plant proteins were subjected to sequential enzymatic digestionin vitro with pepsin, pancreatin + trypsin and erepsin and the release of amino acids as small (including dipeptides) and large peptides and free amino acids, was determined. The relative protein values of α-lactalbumin, egg whites, casein, gluten, zein and protein isolates of soyabeans and groundnuts was determined using rat growth method. It was observed that relative protein value were positively correlated with the essential amino acid index of protein, quantity of essential amino acids released as small peptides and the dipeptide content of enzymatic digests, while there was a negative correlation between relative protein value and essential amino acid content of large peptide fraction.
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Abbreviations
- AA:
-
Amino acid
- EAA:
-
essential AA
- EAAI:
-
EAA index
- PDR:
-
pepsin digest residue
- PPDD:
-
pepsin pancreatin digest dialysate
- PER:
-
protein efficiency ratio
- NPU:
-
net protein utilization
- NPR:
-
net protein retention
- SPI:
-
soya protein isolate
- GNPI:
-
groundnut protein isolate
- AN:
-
amino nitrogen
References
Adibi, S. A. (1971)J. Clin. Invest.,50, 2266.
Akeson, W. R. and Stahman, M. A. (1964)J. Nutr.,83, 257.
Amiot, J., Brisson, G. J., Delisle, J., Goulet, G., Savoie, L. and Jones, J. D. (1980)Nutr.. Int.,24, 513.
Block, R. J. and Mitchell, H. H. (1946)Nutr. Abst. Rev.,16, 249.
Bodwell, C. E. (1981) inProtein Quality in Humans: Assessment and in vitro Estimation (eds C.E. Bodwell, J. S. Adkins and D. T. Hopkins) (Westport, Connecticut: AVI Publ. Co.,) p. 340.
Chen, M. L, Rogers, Q. R. and Harper, A. E. (1962)J. Nutr. 76, 235.
Chung, Y. C., Kim, Y. S., Shadchehr, A., Garrido, A., MacGregor, I. L. and Sleisenger, M. H. (1979)Gastroenterol,76, 1415.
Cossack, Z. T. and Weber, C. W. (1983)Nutr. Rep. Int.,28, 203.
Evaluation of protein quantity (1963) NAS-NRC Publ. No. 1100.
Fazakerley, S. and Best, D. R. (1965)Anal. Biochem.,12, 290.
Ford, J. E. and Salter, D. N. (1966)Br. J. Nutr.,20, 843.
Fukushima, D. (1968)Cereal Chem.,45, 203.
Green, G. M., Olds, B. A., Matthews, G. and Lyman, R. L. (1973)Proc. Soc. Exp. Biol. Med.,142, 1162.
Hegsted, D. M., Neff, R. and Worcester, J. (1968)J. Agric. Food Chem.,16, 192.
Jewell, D. K., Kendrick, J. G. and Satterlee, L. D. (1980)Nutr. Rep. Int.,21, 25.
Kakade, M. L., Hoffa, D. E. and Liener, I. E. (1973)J. Nutr.,103, 1772.
Marable, N. L. and Sanzone, G. (1981) inProtein Quality in Humans: Assessment and in vitro estimation (eds C. E. Bodwell, J. S. Adkins and D. T. Hopkins) (Westport, Connecticut: AVI Publ. Co.,) p. 261.
Marshall, H. F., Jr., Wallace, G. W. and Satterlee, L. D. (1979)Nutr. Rep. Int.,19, 901.
Mauron, J. (1973) inProteins in Human Nutrition (eds J. W. G. Porter, and B. A. Rolls) (London: A. P.) p. 139.
Matthews, D. M., Lis, M. T., Cheng, B. and Crampton, R. F. (1969)Clin. Sci.,37, 751.
Melnick, D., Oser, B. L. and Weiss, S. (1946)Science 103, 326.
Methods of analysis (1975) (ed. W. Horowitz) AOAC 12th Edn. p. 857.
Oser, B. L. (1951)J. Am. Dietet. Assoc.,27, 396.
Pellet, P. L. and Young, V. R. (eds) (1980)Nutritional Evaluation of Protein Foods. (U. N. University Publ.) p. 110.
Pope, H. O., Patten, J. R. and Lawrence, A. L. (1975)Br. J. Nutr.,33, 117.
Raghunath, M. and Narasinga Rao, B. S. (1983)Indian J. Biochem. Biophys.,20, 149.
Ramachandra, G., Virupaksha, T. K. and Shadaksharaswamy, M. (1977)J. Agric. Food. Chem.,25, 1101.
Rich, N., Satterlee, L. D. and Smith, J. L. (1980)Nutr. Rep. Int.,21, 285.
Samonds, K. W. and Hegsted, D. M. (1976) inEvaluation of Protein Foods (Revision of Publ. 1100) NAS, Washington D. C.
Satterlee, L. D., Kendrick, J. G., Jewell, D. K. and Brown, W. D. (1981) inProtein Quality in Humans: Assessment and in vitro estimation (eds C. E. Bodwell, J. S. Adkins and D. T. Hopkins) (AVI Publ. USA) p. 316.
Satterlee, L. D., Marshall, H. F. and Tennyson, J. M. (1979)J. A. O. C. S.,56, 103.
Sheffner, A. L., Eckfeldt, G. A. and Spector, H. (1956)J. Nutr. 60, 105.
Sheffner, A. L. (1967) in Newer methods of Nutritional Biochemistry, (ed. A. A. Albanese) (AP, NY) Vol. 3, p. 125.
Shotton, D. M. and Hartley, B. S. (1970)Nature (London) 225, 802.
Stahman, M. A. and Woldegiorgis, G. (1975) inProtein nutritional quality of foods and feeds part 1 (ed. M. Friedman) (New York: Marcel Dekker INC,) p. 211.
Staples, R. C., Womack, M., Vaughan, D. A. and Bodwell, C. E. (1979)Fed. Proc.,38, 284 (Ab. No. 314).
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Raghunath, M., Rao, B.S.N. Relationship between relative protein value and somein vitro indices of protein quality. J. Biosci. 6, 655–661 (1984). https://doi.org/10.1007/BF02702707
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DOI: https://doi.org/10.1007/BF02702707