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An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton

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Abstract

A comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through an optimized high-performance liquid chromatography (HPLC) on a C18 column (4.6 mm x 250 mm, 5 Μm particle) with methanol-0.5% acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and UV detection at 254 nm. The method was shown to be highly reproducible, with precision [as relative standard deviation (RSD)] and accuracy [as relative mean error (RME)] < 10%, both intra-day and inter-day. Absolute recoveries were > 94%. The results revealed major differences among the different gland varieties or species of cotton, including the special and ordinary glandless and glandedGossypium hirsutum, G. barbadense, and displayed the precious resources of different glands of extraordinary cotton

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Abbreviations

HPLC:

High-performance liquid chromatography

ICIS:

Industrial Crop Institute of Sichuan Academy of Sciences

RME:

relative mean error

RSD:

relative standard error

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Authors and Affiliations

  1. College of Bioinformation, Chongqing University of Post and Telecom, 400065, Chongqing, People’s Republic of China

    Yingfan Cai & Jianchuan Mo

  2. College of Life Science, Sichuan University, 610064, Chengdu, People’s Republic of China

    Yingfan Cai, Hong Zhang, Yu Zeng, Jinku Bao, Chen Miao, Jie Bai, Fang Yan & Fang Chen

Authors
  1. Yingfan Cai
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  2. Hong Zhang
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  3. Yu Zeng
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  4. Jianchuan Mo
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  5. Jinku Bao
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  6. Chen Miao
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  7. Jie Bai
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  8. Fang Yan
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  9. Fang Chen
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Corresponding author

Correspondence to Fang Chen.

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Cai, Y., Zhang, H., Zeng, Y. et al. An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton. J Biosci 29, 67–71 (2004). https://doi.org/10.1007/BF02702563

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  • DOI: https://doi.org/10.1007/BF02702563

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