Skip to main content
SpringerLink
Log in

Epigenetic reprogramming of nuclei using cell extracts

  • Published:
Stem Cell Reviews Aims and scope Submit manuscript

Abstract

Recent evidence indicates that nuclear and cytoplasmic extracts from undifferentiated cells can reprogram gene expression and promote pluripotency in otherwise more developmentally restricted cell types. Notably, extracts of embryonal carcinoma cells or embryonic stem cells have been shown to elicit a shift in the transcriptional program of target cells to upregulate embryonic stem cell genes, downregulate somatic cell-specific markers, and epigenetically modify histones. Reprogrammed kidney epithelial cells acquire a potential for differentiation toward ectodermal and mesodermal lineages. Cell extract-mediated nuclear reprogramming may constitute an attractive alternative to reprogramming somatic cells by cell fusion or nuclear transfer. This review highlights recent observations leading to the concept that extracts derived from pluripotent cells contain regulatory components capable of reprogramming somatic nuclear function. Limitations of current extract-based reprogramming approaches are also addressed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cibelli JB, Stice SL, Golueke PJ, et al. Nat Biotechnol 1998;16:642–646.

    Article  PubMed  CAS  Google Scholar 

  2. Gurdon JB, Byrne JA. Proc Natl Acad Sci USA 2003;100:8048–8052.

    Article  PubMed  CAS  Google Scholar 

  3. Tada M, Tada T, Lefebvre L, Barton SC, Surani MA. EMBO J 1997;16:6510–6520.

    Article  PubMed  CAS  Google Scholar 

  4. Cowan CA, Atienza J, Melton DA, Eggan K. Science 2005; 309:1369–1373.

    Article  PubMed  CAS  Google Scholar 

  5. Tada M, Takahama Y, Abe K, Nakastuji N, Tada T. Curr Biol 2001;11:1553–1558.

    Article  PubMed  CAS  Google Scholar 

  6. Collas P, Håkelien AM. Trends Biotechnol 2003;21:354–361.

    Article  PubMed  CAS  Google Scholar 

  7. Taranger CK, Noer A, Sorensen AL, Hakelien AM, Boquest AC, Collas P. Mol Biol Cell 2005;16:5719–5735.

    Article  PubMed  CAS  Google Scholar 

  8. Qin M, Tai G, Collas P, Polak JM, Bishop AE. Stem Cells 2005; 23:712–718.

    Article  PubMed  CAS  Google Scholar 

  9. Ying QL, Nichols J, Evans EP, Smith AG. Nature 2002;416: 545–548.

    Article  PubMed  CAS  Google Scholar 

  10. Pells S, Di Domenico AI, Callagher EJ, McWhir J. Cloning Stem Cells 2002;4:331–338.

    Article  PubMed  CAS  Google Scholar 

  11. Terada N, Hamazaki T, Oka M, et al. Nature 2002;416:542–545.

    Article  PubMed  CAS  Google Scholar 

  12. Flasza M, Shering AF, Smith K, Andrews PW, Talley P, Johnson PA. Cloning Stem Cells 2003;5:339–354.

    Article  PubMed  CAS  Google Scholar 

  13. Kimura H, Tada M, Nakatsuji N, Tada T. Mol Cell Biol 2004;24: 5710–5720.

    Article  PubMed  CAS  Google Scholar 

  14. Håkelien AM, Landsverk HB, Robl JM, Skålhegg BS, Collas P. Nat Biotechnol 2002;20:460–466.

    Article  PubMed  Google Scholar 

  15. Gaustad KG, Boquest AC, Anderson BE, Gerdes AM, Collas P. Biochem Biophys Res Commun 2004;314:420–427.

    Article  PubMed  CAS  Google Scholar 

  16. McGann CJ, Odelberg SJ, Keating MT. Proc Natl Acad Sci USA 2001;98:13,699–13,704.

    Article  CAS  Google Scholar 

  17. Hansis C, Barreto G, Maltry N, Niehrs C. Curr Biol 2004;14: 1475–1480.

    Article  PubMed  CAS  Google Scholar 

  18. Collas P, Pinto-Correia C, Poccia DL. Exp Cell Res 1995;219: 687–698.

    Article  PubMed  CAS  Google Scholar 

  19. Collas P, Poccia DL. J Cell Sci 1996;109:1275–1283.

    PubMed  CAS  Google Scholar 

  20. Lohka MJ, Masui Y. Science 1983;220:719–721.

    Article  PubMed  CAS  Google Scholar 

  21. Lohka MJ, Masui Y. J Cell Biol 1984;98:1222–1230.

    Article  PubMed  CAS  Google Scholar 

  22. Ulitzur N, Gruenbaum Y. FEBS Lett 1989;259:113–116.

    Article  PubMed  CAS  Google Scholar 

  23. Wilson KL, Newport JW. J Cell Biol 1988;107:57–68.

    Article  PubMed  CAS  Google Scholar 

  24. Collas, P, Le Guellec K, Tasken K. J Cell Biol 1999;147:1167–1180.

    Article  PubMed  CAS  Google Scholar 

  25. Eide T, Carlson C, Tasken KA, Hirano T, Tasken K, Collas P. EMBO Rep 2002;3:426–432.

    Article  PubMed  CAS  Google Scholar 

  26. Landsverk HB, Kirkhus M, Bollen M, Kuntziger T, Collas P. Biochem J 2005;390:709–717.

    PubMed  CAS  Google Scholar 

  27. Martins SB, Eide T, Steen RL, Jahnsen T, Skålhegg BS, Collas P. J Cell Sci 2000;113:3703–3713.

    PubMed  CAS  Google Scholar 

  28. Steen RL, Martins SB, Tasken K, Collas P. J Cell Biol 2000;150:1251–1262.

    Article  PubMed  CAS  Google Scholar 

  29. Håkelien AM, Gaustad KG, Collas P. Meth Cell Biol 2005; 325:99–114.

    Google Scholar 

  30. Håkelien AM, Gaustad KG, Taranger CK, Skålhegg BS, Kuntziger T, Collas P. Exp Cell Res 2005;309:32–47.

    Article  PubMed  Google Scholar 

  31. Håkelien AM, Gaustad KG, Collas P. Biochem Biophys Res Commun 2004;316:834–841.

    Article  PubMed  Google Scholar 

  32. Wilmut I, Beaujean N, De Sousa PA, et al. Nature 2002; 419:583–586.

    Article  PubMed  CAS  Google Scholar 

  33. Byrne JA, Simonsson S, Western PS, Gurdon JB. Curr Biol 2003;13:1206–1213.

    Article  PubMed  CAS  Google Scholar 

  34. Simonsson S, Gurdon J. Nat Cell Biol 2004;6:984–990.

    Article  PubMed  CAS  Google Scholar 

  35. Liu Y, Shin S, Zeng X, et al. BMC Dev Biol 2006;6:20.

    Article  PubMed  Google Scholar 

  36. Sperger JM, Chen X, Draper JS, et al. Proc Natl Acad Sci USA 2003;100:13,350–13,355.

    Article  CAS  Google Scholar 

  37. Blelloch RH, Hochedlinger K, Yamada Y, et al. Proc Natl Acad Sci USA 2004;101:13,985–13,990.

    CAS  Google Scholar 

  38. Miklos GL, Maleszka R. Nat Biotechnol 2004;22:615–621.

    Article  PubMed  CAS  Google Scholar 

  39. Mathieu O, Bender J. J Cell Sci 2004;117:4881–4888.

    Article  PubMed  CAS  Google Scholar 

  40. Matzke MA, Birchler JA. Nat Rev Genet 2005;6:24–35.

    Article  PubMed  CAS  Google Scholar 

  41. Nishimoto M, Fukushima A, Okuda A, Muramatsu M. Mol Cell Biol 1999;19:5453–5465.

    PubMed  CAS  Google Scholar 

  42. Boquest AC, Shahdadfar A, Fronsdal K, et al. Mol Biol Cell 2005;16:1131–1141.

    Article  PubMed  CAS  Google Scholar 

  43. Akashi K, He X, Chen J, et al. Blood 2003;101:383–389.

    Article  PubMed  CAS  Google Scholar 

  44. Woodbury D, Reynolds K, Black IB. J Neurosci Res 2002;69: 908–917.

    Article  PubMed  CAS  Google Scholar 

  45. Grunau C, Clark SJ, Rosenthal A. Nucleic Acids Res 2001;29:E65.

    Article  PubMed  CAS  Google Scholar 

  46. Warnecke PM, Stirzaker C, Song J, Grunau C, Melki JR, Clark SJ. Methods 2002;27:101–107.

    Article  PubMed  CAS  Google Scholar 

  47. Nordhoff V, Hubner K, Bauer A, Orlova I, Malapetsa A, Scholer HR. Mamm Genome 2001;12:309–317.

    Article  PubMed  CAS  Google Scholar 

  48. Ramchandani S, Bhattacharya SK, Cervoni N., Szyf M. Proc Natl Acad Sci USA 1999;96:6107–6112.

    Article  PubMed  CAS  Google Scholar 

  49. Morgan HD, Dean W, Coker HA, Reik W, Petersen-Mahrt SK. J Biol Chem 2004;279:52,353–52,360.

    CAS  Google Scholar 

  50. Kikyo N, Wade PA, Guschin D, Ge H, Wolffe AP. Science 2000;289:2360–2362.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, Blindern, PO Box 1112, 0317, Oslo, Norway

    Philippe Collas & Christel K. Taranger

Authors
  1. Philippe Collas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christel K. Taranger
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Philippe Collas.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Collas, P., Taranger, C.K. Epigenetic reprogramming of nuclei using cell extracts. Stem Cell Rev 2, 309–317 (2006). https://doi.org/10.1007/BF02698058

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02698058

Index Entries

Search

Navigation