Skip to main content

Advertisement

SpringerLink
Log in

Hybridization of 5′-end-labelled RNA to plant viral RNA in agarose and acrylamide gels

  • Experimental Protocols
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Alwine, J.C., D.J. Kemp, and G.R. Stark, (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc. Natl. Acad. Sci. USA 74:5350–5354.

    Article  PubMed  CAS  Google Scholar 

  • Bailey, J.M. and N. Davidson (1976). Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal. Biochemistry 70:75–85.

    Article  CAS  Google Scholar 

  • Condit, C., and H. Fraenkel-Conrat (1979). Isolation of replicative forms of 3'terminal subgenomic RNAs of tobacco necrosis virus. Virolog 97:122–130.

    Article  CAS  Google Scholar 

  • Dawson, W.O. and J.A. Dodds (1982). Characterization of sub-genomic double-stranded RNAs from virus infected plants. Biochem. Biophys. Res. Commun. 107:1230–1235.

    Article  PubMed  CAS  Google Scholar 

  • Dodds, J.A. and M. Bar-Joseph (1983). Double-stranded RNA from plants infected with closteroviruses. Phytopathol. in press.

  • Gooding, G.V. and T.T. Hebert (1967). A simple technique for purification of tobacco mosaic virus in large quantities. Phytopathol. 57:1285.

    Google Scholar 

  • Goelet, P. and J. Karn (1982). Tobacco mosaic virus induces the synthesis of a family of 3′ coterminal messenger RNAs and their complements. J. Mol. Biol. 154:541–550.

    Article  PubMed  CAS  Google Scholar 

  • Grill, L.K. and S.J. Garger (1981). Identification and characterization of double-stranded RNA associated with cytoplasmic male sterility inVicia faba. Proc. Natl. Acad. Sci. (USA) 78:7043–7046.

    Article  CAS  Google Scholar 

  • Henriques, M.I.C. and T.J. Morris (1979). Evidence for different replicative strategies in the plant tombusviruses. Virology 99, 66–74.

    Article  CAS  Google Scholar 

  • Jordan, R.L., J.A. Dodds, and H.D. Ohr (1983). Evidence for virus-like agents in avocado. Phytopathol. in press.

  • Morris, T.J. and J.A. Dodds (1979). Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathol. 69:854–858.

    CAS  Google Scholar 

  • Negruk, V.J., L.K. Grill, and J.S. Semancik (1980).In vitro 32P-labelling of viroid RNA for hybridization studies. J. Virol. Meth. 1:229–234.

    Article  CAS  Google Scholar 

  • Ralph, R.K. (1969). Double-stranded viral RNA. Adv. Virus Res. 15:61–158.

    PubMed  CAS  Google Scholar 

  • Rosner, A., M. Bar-Joseph, M. Moscovitz, and M. Mevarech (1983). Diagnosis of specific viral RNA sequences in plant extracts by hybridization with a polynucleotide kinase labelled double-stranded RNA probe. Phytopathol. in press.

  • Shinnick, T.M., E. Lund, O. Smithies, and F.R. Blattner (1975). Hybridization of labelled RNA to DNA in agarose gels. Nucl. Acid Res. 2:1911–1929.

    Article  CAS  Google Scholar 

  • Southern, E.M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503–517.

    Article  PubMed  CAS  Google Scholar 

  • Stellwag, E.J. and A.E. Dahlberg (1980). Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethol (DBM)-paper. Nucl. Acid. Res. 2:299–317.

    Article  Google Scholar 

  • Thomas, P. (1980). Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77:5201–5205.

    Article  PubMed  CAS  Google Scholar 

  • Tsao, S., C. Brunk, and R. Pearlman (1981). Hybridizing dry agarose gels. Plant Mol. Biol. Newsletter 2:100–101.

    Google Scholar 

  • Zelcer, A., K.F. Weaber, E. Balaza, and M. Zaitlin (1981). The detection and characterization of viral-related double-stranded RNAs in tobacco mosaic virus-infected plants. Virology 113:417–427.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Pathology, University of California, 92521, Riverside, CA, USA

    Ramon L. Jordan & J. Allan Dodds

Authors
  1. Ramon L. Jordan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Allan Dodds
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jordan, R.L., Dodds, J.A. Hybridization of 5′-end-labelled RNA to plant viral RNA in agarose and acrylamide gels. Plant Mol Biol Rep 1, 31–37 (1983). https://doi.org/10.1007/BF02680260

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02680260

Keywords

Search

Navigation