Abstract
Lipids in soybean defatted meal, concentrate and isolate were extracted by four procedures: (a) a Soxhlet extract by chloroform-methanol; (b) a Soxhlet extraction by benzene/ethanol; (c) a short extraction by chloroform/methanol; and (d) a short extraction by hexane/ethanol. Procedure 2 extracted more lipid than Procedure 1 from the isolate and meal. Both Soxhlet procedures extracted more lipid than Procedure 3 from the meal only, and more lipid than Procedure 4 from all products. Percent lipid on dry matter basis ranged for the meal, 1.56 to 4.52; concentrate 0.90 to 1.44; and isolate, 0.28 to 0.96. Lipids extracted from each product by Procedures 3 and 4 were fractionated quantitatively into (a) neutral lipids, (b) polar lipids except lecithin, and (c) lecithin. Fatty acid (C12–C20) composition of each lipid fraction was determined, and attempts were made to identify lipids. The larger amount of lipid in any product extracted by either Procedure 3 or 4 was in Fraction 1. Linoleic acid was the most abundant acid found in any lipid fraction. Significantly more oleic acid was found in Fractions I and II Concentrate lipids than in the same fractions of meal or isolate lipids. A number of lipids were found in Fractions I and II, but the only lipid in Fraction III was lecithin.
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Melton, S.L., Moyers, R.E. & Playford, C.G. Lipids extracted from soy products by different procedures. J Am Oil Chem Soc 56, 489–493 (1979). https://doi.org/10.1007/BF02680185
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DOI: https://doi.org/10.1007/BF02680185