Abstract
We describe a western blotting procedure in which immobilized DNA is reacted with monoclonal antibodies specific to individual types of DNA damage. This method is applicable to small DNA samples and is more sensitive than the standard assay.
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Abbreviations
- CPD:
-
cyclobutane pyrimidine dimer
- HRP:
-
horse-radish peroxidase
- TEV:
-
endonuclease V from phage T4.PBS andPBS-T are defined inMaterials and Solutions
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Stapleton, A.E., Mori, T. & Walbot, V. A simple and sensitive antibody-based method to measure UV-induced DNA damage inZea mays . Plant Mol Biol Rep 11, 230–236 (1993). https://doi.org/10.1007/BF02669850
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DOI: https://doi.org/10.1007/BF02669850