Abstract
After many unsuccessful attempts to obtain biologically active mRNAs from spruce (Picea abies) tissues using available protocols, we have adapted a procedure for the isolation of RNAs from needles, shoots, and callus ofPicea species. Our modifications permit the recovery of and an average of 300 μg RNA per g of needles that is suitable for translationin vitro, northern hybridizations, and the construction of cDNA libraries.
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Bahloul, M., Burkard, G. An improved method for the isolation of total RNA from spurce tissues. Plant Mol Biol Rep 11, 212–215 (1993). https://doi.org/10.1007/BF02669847
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DOI: https://doi.org/10.1007/BF02669847