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A rapid and efficient method for the extraction of total DNA from the sweet potato and its related species

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Abstract

Secondary metabolites, latex/mucilagenous secretions, polysaccharides, and proteins interfere with the extraction of high-quality, restrictable total cellular DNA from sweet potato [Ipomoea batatas (L.) Lamk.] and related species. A method for the DNA extraction is described which overcomes these problems.

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Abbreviations

CTAB:

hexadecyltrimethylammonium bromide

RFLP:

restriction fragment length polymorphism

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Authors and Affiliations

  1. Plant Molecular and Cellular Genetics Laboratory, School of Agriculture, Tuskegee University, 36088, Tuskegee, AL, USA

    G. S. Varadarajan & C. S. Prakash

Authors
  1. G. S. Varadarajan
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  2. C. S. Prakash
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Varadarajan, G.S., Prakash, C.S. A rapid and efficient method for the extraction of total DNA from the sweet potato and its related species. Plant Mol Biol Rep 9, 6–12 (1991). https://doi.org/10.1007/BF02669284

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  • DOI: https://doi.org/10.1007/BF02669284

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