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Mini-preparation of highly purified chloroplast DNA from maize

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Abbreviations

cpDNA:

chloroplast DNA

EDTA:

ethylenediaminotetraacetate

References

  • Bogorad, L., E.J. Gubbins, E. Krebbers, I.M. Larrinau, B.J. Mulligan, K.M.T. Muskuvitch, E.A. Orr, S.R. Rodermel, R. Schantz, A.A. Steinmetz, G. De Vos, V. K. Ye. 1983. Cloning and physical mapping of maize plastid genes. In: Meth. Enzmol. 97: 524–555.

  • Feinberg, A. and B. Vogelstein. 1984. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137: 266–267.

    Article  PubMed  CAS  Google Scholar 

  • Herrmann, R.G., H.J. Bohnert, K.V. Kowallik, J.M. Schmitt. 1975. Size, conformation and purity of chloroplast DNA from some higher plants. Biochim. Biophys. Acta 378: 305–317.

    PubMed  CAS  Google Scholar 

  • Jackson, P.J. and K.G. Lark. 1982. Ribosomal RNA synthesis in soybean suspension cultures growing in different media. Plant Physiol. 69: 234–239.

    Article  PubMed  CAS  Google Scholar 

  • Kolodner, R. and K.K. Tewari. 1975. The molecular size and conformation of the chloroplast DNA from higher plants. Biochim. Biophys. Acta 402: 372–390.

    PubMed  CAS  Google Scholar 

  • Mourad, G. 1987. Homoplastidic Mutants of Arabidopsis, Ph.D. Thesis, University of Missouri, Columbia, MO.

    Google Scholar 

  • Murray, M.G. and W.F. Thompson. 1980. Rapid isolation of high molecular weight plant DNA. Nucl. Acids. Res. 8: 4321–4325.

    Article  PubMed  CAS  Google Scholar 

  • Palmer, J.D. 1986. Isolation and structural analysis of chloroplast DNA. Meth. Enzymol. 118: 167–186.

    Article  CAS  Google Scholar 

  • Rogers, S.O. and A.J. Bendich. 1987. Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. Plant Molec. Biol. 9: 509–520.

    Article  CAS  Google Scholar 

  • Westhoff, P., J. Alt, N. Nelson, W. Bottomley, H. Bünemann and R.G. Herrmann. 1983. Genes and transcripts for the P700 chlorophyla apoprotein and subunit 2 of the photosystem I reaction center complex from spinach thylakoid membranes. Plant Mol. Biol. 2: 95–107.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Biochemistry Department, University of Missouri, 65211, Columbia, MO, USA

    George Mourad & Mary L. Polacco

Authors
  1. George Mourad
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  2. Mary L. Polacco
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Erratum: This is a corrected version of an article that appeared in thePlant Molecular Biology Reporter 6(3): 193–199.

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Mourad, G., Polacco, M.L. Mini-preparation of highly purified chloroplast DNA from maize. Plant Mol Biol Rep 7, 78–84 (1989). https://doi.org/10.1007/BF02669249

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  • DOI: https://doi.org/10.1007/BF02669249

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