Skip to main content
SpringerLink
Log in

GUS activity and generation of transformed shoot buds are highly correlated inAgrobacterium-transformed tobacco

  • Commentary
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

4-MU:

4-methylumbelliferone

4-MUG:

4-methylumbelliferyl-β-D-glucuronide

GUS:

β-glucuronidase

MS:

Murashige-Skoog

uidA :

gene encoding β-glucuronidase.

References

  • An, G., P.R. Ebert, A. Mitra and S.B. Ha. 1988. Binary vectors, pp. A3/1–19 inPlant Molecular Biology Manual (eds. S. B. Gelvin and R. A. Schilperoort). Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Breyne, P., M.D. Loose, A. Didonder, M. Van Montagu and A. Depicker. 1993. Quantitative kinetic analysis of β-glucuronidase activities using a computer-directed microtiter plate reader. Plant Mol. Biol. Reptr. 11:21–31.

    Article  CAS  Google Scholar 

  • Castle, L.A. and R.O. Morris. 1990. A method for early detection of T-DNA transfer. Plant Mol. Biol. Reporter 8:28–39.

    CAS  Google Scholar 

  • DeBlock, M., J. Botterman, M. Vandewiele, J. Dockx, C. Thoen, V. Gossele, V. Rao Movva, C. Thompson, M. Van Montagu and J. Leemans. 1987. Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J. 6:2513–2518.

    CAS  Google Scholar 

  • Ditta, G., S. Stanfield, D. Corbin and D.R. Helinski. 1980. Broad host-range DNA cloning systems for Gram-negative bacteria: Construction of a gene bank ofRhizobium meliloti. Proc. Natl. Acad. Sci. USA 77:7347–7351.

    Article  PubMed  CAS  Google Scholar 

  • Draper, J., R. Scott and J. Hamil. 1988. Transformation of dicotyledonous plant cells using the Ti plasmid ofAgrobacterium tumefaciens and the Ri plasmid ofA. rhizogenes, pp. 69–160. inPlant Genetic Transformation and Gene Expression. A Laboratory Manual. (eds. J. Draper, R. Scott, P. Armitage and R. Walden). Blackwell Scientific Publications, Oxford, UK.

    Google Scholar 

  • Godwin, I., G. Todd, B. Ford-Lloyd and H.J. Newbury. 1991. The effects of acetosyringone and pH onAgrobacterium-mediated transformation vary according to plant species. Plant Cell Reports 9:671–675.

    Article  CAS  Google Scholar 

  • Guerineau, F., L. Brooks, J. Meadows, A. Lucy, C. Robinson and P. Mullineaux. 1990. Sulfonamide resistance gene for plant transformation. Plant Mol. Biol. 15:127–136.

    Article  PubMed  CAS  Google Scholar 

  • Hawes, M.C., S.L. Robbs and S.G. Pueppke. 1989. Use of a root tumorigenesis assay to detect genotypic variation in susceptibility of thirty-four cultivars ofPisum sativum to crown gall. Plant Physiol. 90:180–184.

    Article  PubMed  Google Scholar 

  • Holford, P., N. Hernandez and H.J. Newbury. 1992. Factors influencing the efficiency of T-DNA transfer during co-cultivation ofAntirrhinum majus withAgrobacterium tumefaciens. Plant Cell Reports 11:196–199.

    CAS  Google Scholar 

  • Horsch, R.B., J. Fry, N. Hoffmann, J. Neidermeyer, S.G. Rogers and R.T. Fraley 1988. Leaf disc transformation, pp. A3/1–19 inPlant Molecular Biology Manual. (eds., S.B. Gelvin and R.A. Schilperoort). Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Janssen, B.-J. and R.C. Gardner. 1989. Localized transient expression of GUS in leaf discs following cocultivation withAgrobacterium. Plant Mol. Biol. 14:61–72.

    Article  Google Scholar 

  • Jefferson, R.A., T.A. Kavanagh and M.W. Bevan. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6:3901–3907.

    PubMed  CAS  Google Scholar 

  • Li, X-Q., C-N. Liu, S.W. Ritchie, J.-Y. Peng, S.B. Gelvin and T.K. Hodges. 1992. Factors influencingAgrobacterium-mediated transient expression ofgusA in rice. Plant Mol. Biol. 20:1037–1048.

    Article  PubMed  CAS  Google Scholar 

  • Lippincott, J.A. and G.T. Heberlein. 1965. The quantitative determination of the infectivity ofAgrobacterium tumefaciens. Am. J. Bot. 52:856–863.

    Article  PubMed  CAS  Google Scholar 

  • Minnemeyer, S.L., R. Lightfoot and A.G. Matthysse. 1991. A semiquantitative bioassay for relative virulence ofAgrobacterium tumefaciens strains onBryophyllum daigremontiana. J. Bacteriol. 173:7723–7724.

    PubMed  CAS  Google Scholar 

  • Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497.

    Article  CAS  Google Scholar 

  • Peng, J., F. Wen and T.K. Hodges. 1993. A rapid method for qualitative assay of both neomycin phosphotransferase II and β-glucuronidase activities in transgenic plants. Plant Mol. Biol. Reporter 11:38–47.

    CAS  Google Scholar 

  • Peralta, E.G. and L.W. Ream. 1985. T-DNA border sequences required for crown gall tumorigenesis. Proc. Natl. Acad. Sci. USA 82:5112–5116.

    Article  PubMed  CAS  Google Scholar 

  • Rossi, L., J. Escudero, B. Hohn and B. Tinland. 1993. Efficient and sensitive assay for T-DNA dependent transient gene expression. Plant Mol. Biol. Reporter 11:220–229.

    CAS  Google Scholar 

  • Sangwan, R.S., Y. Bourgeois and B.S. Sangwan-Norreel. 1991. Genetic transformation ofArabidopsis thaliana zygotic embryos and identification of critical parameters influencing transformation efficiency. Mol. Gen. Genet. 230:475–485.

    Article  PubMed  CAS  Google Scholar 

  • Stachel, S.E. and E.W. Nester. 1986. The genetic and transcriptional organization of thevir region of the A6 Ti plasmid ofAgrobacterium tumefaciens. EMBO J. 5:1445–1454.

    PubMed  CAS  Google Scholar 

  • Turk, S.C.H.J., L.S. Melchers, H. den Dulk-Ras, A.J.G. Regensburg Tuink and P.J.J. Hooykaas. 1991. Environmental conditions differentially affectvir gene induction in differentAgrobacterium strains. Role of the virA sensor protein. Plant Mol. Biol. 16:1051–1059.

    Article  PubMed  CAS  Google Scholar 

  • Wang, K., A. Herrera-Estrella and M. Van Montagu. 1990. Over expression ofvirD1 andvirD2 genes inAgrobacterium tumefaciens enhances T-complex formation and plant transformation. J. Bacteriol. 172:4432–4440.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biotechnology Centre, Queens University of Belfast, New Forde Lane, BT9 5PX, Belfast, UK

    K. S. Sarma

  2. Centre for Plant Molecular Biology, School of Biotechnology, Madurai Kamaraj University, 625 021, Madurai, India

    G. Sunilkumar, V. Balamani & K. Veluthambi

Authors
  1. K. S. Sarma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Sunilkumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Balamani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Veluthambi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sarma, K.S., Sunilkumar, G., Balamani, V. et al. GUS activity and generation of transformed shoot buds are highly correlated inAgrobacterium-transformed tobacco. Plant Mol Biol Rep 13, 377–382 (1995). https://doi.org/10.1007/BF02669194

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02669194

Keywords

Search

Navigation