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Production and purification of synthetic peptide antibodies

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References

  • Burnette, W. N. (1981). “Western blotting:” electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.Anal. Biochem. 112: 195–203.

    Article  PubMed  CAS  Google Scholar 

  • Chomyn, A., M. W. J. Cleeter, C. I. Ragan, M. Riley, R. F. Doolittle, and G. Attardi (1986). URF6, last unidentified reading frame of human mtDNA, codes for an NADH dehydrogenase subunit.Science 234: 614–618.

    Article  PubMed  CAS  Google Scholar 

  • Chomyn, A., P. Mariottini, M. W. J. Cleeter, C. I. Ragan, A. Matsuno-Yagi, Y. Hatefi, R. F. Doolittle, and G. Attardii (1985). Six unidentified, reading frames of human mitochondrial DNA encode components of the respiratory-chain NADH dehydrogenase.Nature 314: 592–597.

    Article  PubMed  CAS  Google Scholar 

  • Chomyn, A., P. Mariottini, N. Gonzalez-Cadavid, G. Attardi, D. D. Strong, D. Trovato, M. Riley, and R. F. Doolittle (1983). Identification of the polypeptides encoded in the ATPase 6 gene and in the unassigned reading frames 1 and 3 of human mtDNA.Proc. Natl. Acad. Sci. U.S.A. 80: 5535–5539.

    Article  PubMed  CAS  Google Scholar 

  • Dawson, R. M. C., D. C. Elliot, W. H. Elliot, and K. M. Jones (Eds.) (1974).Data for Biochemical Research, London: Oxford University Press.

    Google Scholar 

  • Engvall, E. (1980). Enzyme immunoassay ELISA and EMIT.Meth. Enzymol. 70: 419–439.

    PubMed  CAS  Google Scholar 

  • Erlanger, B. F. (1980). The preparation of antigenic hapten-carrier conjugates: a survey.Meth. Enzymol. 70: 85–104.

    PubMed  CAS  Google Scholar 

  • Fish, L. E. and L. Bogorad (1986). Identification and analysis of the maize P700 chlorophyll a apoproteins PSI-A1 and PSI-A2 by high pressure liquid chromatography analysis and partial sequence determination.J. Biol. Chem. 261: 8134–8139.

    PubMed  CAS  Google Scholar 

  • Fish, L. E., U. Kuck, and L. Bogorad (1985). Two partially homologous adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyl a-protein complex of photosystem I.J. Biol. Chem. 260: 1413–1421.

    PubMed  CAS  Google Scholar 

  • Garvey, J. S., N. E. Cremer, and D. H. Sussdorf (1977).Methods in Immunology, Reading, MA: W. A. Benjamin, Inc.

    Google Scholar 

  • Green, N., H. Alexander, A. Olson, S. Alexander, T. M. Shinnick, J. G. Sutcliffe, and R. A. Lerner (1982). Immunogenic, structure of the influenza virus hemagglutinin.Cell 28: 477–487.

    Article  PubMed  CAS  Google Scholar 

  • Guiso, N., M. Dreyfus, O. Siffert, A. Danchin, A. Spyridakis, A. Gargouri, M. Claisse, and P. P. Slonimski (1984). Antibodies against synthetic oligopeptides allow identification of the mRNA-maturase encoded by the second intron of yeastcob-box gene.EMBO J. 3: 1769–1772.

    PubMed  CAS  Google Scholar 

  • Houghten, R. A., S. T. DeGraw, M. K. Bray, S. R. Hoffman, and N. D. Frizzell (1986). Simultaneous multiple peptide synthesis: the rapid preparation of large numbers of discrete peptides for biological, immunological, and methodological studies.BioTechniques 4: 522–528.

    CAS  Google Scholar 

  • Johnson, D. A., J. W. Gautsch, J. R. Sportsman, and J. H. Elder (1984). Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose.Gene Anal. Techn. 1: 3–8.

    Article  CAS  Google Scholar 

  • Kyte, J. and R. F. Doolittle (1982). A simple method for displaying the hydropathic character of a protein.J. Mol. Biol. 157: 105–132.

    Article  PubMed  CAS  Google Scholar 

  • Lerner, R. A. (1982). Tapping the immunological repertoire to produce antibodies of predetermined specificity.Nature 299: 592–596.

    Article  CAS  Google Scholar 

  • Lerner, R. A. (1984). Antibodies of predetermined specificity in biology and medicine.Advances in Immunology 36: 1–44.

    PubMed  CAS  Google Scholar 

  • Liu, F.-T., M. Zinnecker, T. Hamaoka, and D. H. Katz (1979). New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates.Biochemistry 18: 690–697.

    Article  PubMed  CAS  Google Scholar 

  • Mariottini, P., A. Chomyn, G. Attardi, D. Trovato, D. D. Strong, and R. F. Doolittle (1983). Antibodies against synthetic peptides reveal that the unidentified reading frame A6L, overlapping the ATPase 6 gene, is expressed in human mitochondria.Cell 32: 1269–1277.

    Article  PubMed  CAS  Google Scholar 

  • Mariottini, P., A. Chomyn, M. Riley, B. Cottrell, R. F. Doolittle, and G. Attardi (1986). Identification of the polypeptides encoded in the unassigned reading frames 2, 4, 4L, and 5 of human mitochondrial DNA.Proc. Natl. Acad. Sci. U.S.A. 83: 1563–1567.

    Article  PubMed  CAS  Google Scholar 

  • Michael, N. L., J. B. Rothbard, R. A. Shiurba, H. K. Linke, G. K. Schoolnik, and D. A. Clayton (1984). All eight unassigned reading frames of mouse mitochondrial DNA are expressed.EMBO J. 3: 3165–3175.

    PubMed  CAS  Google Scholar 

  • Ohyama, K., H. Fukuzawa, T. Kohchi, H. Shirai, T. Sano, S. Sano, K. Umesono, Y. Shiki, M. Takeuchi, Z. Chang, S. Aota, H. Inokuchi, and H. Ozeki (1986). Chloroplast gene organization deduced from complete sequence of liverwortMarchantia polymorpha chloroplast DNA.Nature 322: 572–574.

    Article  CAS  Google Scholar 

  • Reichlin, M. (1980). Use of glutaraldehyde as a coupling agent for proteins and peptides.Meth. Enzymol. 70: 159–165.

    Article  PubMed  CAS  Google Scholar 

  • Shinozaki, K., M. Ohme, M. Tanaka, T. Wakasugi, N. Hayashida, T. Matsubayashi, N. Zaita, J. Chunwongse, J. Obokata, K. Yamaguchi-Shinozaki, C. Ohto, K. Torazawa, B. Y. Meng, M. Sugita, H. Deno, T. Kamogashira, K. Yamada, J. Kusuda, F. Takaiwa, A. Kato, N. Tohdoh, H. Shimada, and M. Sugiura (1986). The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.EMBO J. 5: 2043–2049.

    PubMed  CAS  Google Scholar 

  • Stewart, J. M. (1983). Synthesis and use of neuropeptides.Meth. Enzymol. 103: 3–32.

    PubMed  CAS  Google Scholar 

  • Towbin, H., T. Staehelin, and J. Gordon (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proc. Natl. Acad. Sci. U.S.A. 76: 4350–4354.

    Article  PubMed  CAS  Google Scholar 

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  1. Division of Biological Sciences, Section of Genetics and Development, Cornell University, 14853, Ithaca, NY

    Helen T. Nivison & Maureen R. Hanson

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  1. Helen T. Nivison
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  2. Maureen R. Hanson
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Nivison, H.T., Hanson, M.R. Production and purification of synthetic peptide antibodies. Plant Mol Biol Rep 5, 295–309 (1987). https://doi.org/10.1007/BF02668993

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