Skip to main content
SpringerLink
Log in

Protein slot blotting: An easy, rapid and reliable technique to identify the expression of a protein in transgenic plants

  • Protocol
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

A technique based on immunological recognition of a foreign protein in transgenic plants has been developed. It allows a quick and reliable screening of many plant samples, improves the accuracy of the results compared to ELISA and is easier to carry out and more sensitive than a western immunoblot. This technique has also been tested to recognize foreign proteins in rice and tobacco leaf extracts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Lapidot, M., Gafny, R., Ding, B., Wolf, S., Lucas, W. L. and Beachy, R. N. 1993. A dysfunctional movement protein of tobacco mosaic virus that partially modifies the plasmodesmata and limits virus spread in transgenic plants. Plant J.4:959–970.

    Article  CAS  Google Scholar 

  • Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989.Molecular Cloning. A Laboratory Manual (second edition). Cold Spring Harbor Laboratory Press.

Download references

Author information

Authors and Affiliations

  1. Division of Plant Biology, The Scripps Research Institute, Iltab/orstom International Laboratory for Tropical Agricultural Biotechnology, 10666 North Torrey Pines Road, 92037, La Jolla, CA, USA

    Alexandre de Kochko, Philippe Marmey, Iann Rance & Claude Fauquet

  2. Division of Plant Biology, The Scripps Research Institute, Iltab/tsri: International laboratory for Tropical Agricultural Biotechnology, 10666 North Torrey Pines Road, 92037, La Jolla, CA, USA

    Rongda Qu & Roger N. Beachy

Authors
  1. Alexandre de Kochko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rongda Qu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Philippe Marmey
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Iann Rance
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Claude Fauquet
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Roger N. Beachy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

de Kochko, A., Qu, R., Marmey, P. et al. Protein slot blotting: An easy, rapid and reliable technique to identify the expression of a protein in transgenic plants. Plant Mol Biol Rep 12, 274–278 (1994). https://doi.org/10.1007/BF02668750

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02668750

Key Words

Search

Navigation