Abstract
A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning.
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Abbreviations
- PFGE:
-
pulsed-field gel electrophoresis
- YAC:
-
yeast artificial chromosome
- BAC:
-
bacterial artificial chromosome
References
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Zhao, X., Zhang, HB., Wing, R.A. et al. A simple method for isolation of megabase DNA from cotton. Plant Mol Biol Rep 12, 110–115 (1994). https://doi.org/10.1007/BF02668372
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DOI: https://doi.org/10.1007/BF02668372