Summary
Nodulated plants of field pea (Pisum arvense L.) were grown for 4 weeks in a range of constantly maintained levels of nitrate labelled with N15. The total and labelled nitrogen in soluble and insoluble fractions of different parts of the treated plants were determined, and from such measurements the distribution of the nitrogen assimilated from nitrate and from the atmosphere was assessed.
Acetylene reduction by whole nodulated roots, and the activity of the nitrate reductase enzyme extracted from various organs of the host plant were monitored at frequent intervals during the same four-week growth period of the treated plants, and by compounding the results from these assays it was possible to calibrate each of them against appropriate measurements of assimilation using the labelled nitrogen. The usefulness of these techniques in studying the plant response to nitrate is discussed.
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Oghoghorie, C.G.O., Pate, J.S. The nitrate stress syndrome of the nodulated field pea (Pisum arvense L.) Techniques for measurement and evaluation in physiological terms. Plant Soil 35, 185–202 (1971). https://doi.org/10.1007/BF02661850
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DOI: https://doi.org/10.1007/BF02661850