Abstract
Biological problems involving long chain compounds and steroids can now be studied more effectively than ever before through use of GLC techniques. When combined with TLC methods for the separation of classes of compounds, these procedures are the most valuable analytical methods now known for lipid investigations. The fact that both qualitative and quantitative data may be obtained at the same time, and that the methods may be used to study complex mixtures at the microgram and sub-microgram level, suggests that many new applications will be found in the fields of chemistry, biology and medicine.
The procedures described in this paper are chiefly those developed over the last few years in the laboratories of the authors. Thin-film columns prepared with diatomaceous earth supports have been used since 1960 in many studies. A new procedure, utilizing a thin-film column, was developed for the separation and estimation of long chain fatty acid methyl esters. The method has been used in conjuction with TLC separations using silica gel and silica gel-silver nitrate plates. The relationship between retention time behavior and the structure of steroids has been studied, and the “steroid number” concept has been used to described GLC properties of steroids. Procedures have been found for the inactivation of supports, for the modification of liquid phase properties, for the preparation of a variety of special derivatives useful in GLC work and for the study of several groups of steroids important in human metabolism. A number of quantitative analytical separations were also developed.
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Horning, E.C., Vandenheuvel, W.J.A. Gas chromatography in lipid investigations. J Am Oil Chem Soc 41, 707–716 (1964). https://doi.org/10.1007/BF02661415
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DOI: https://doi.org/10.1007/BF02661415