Skip to main content
SpringerLink
Log in

Silver-complexation liquid chromatography for fast, high-resolution separations of triacylglycerols

  • Published:
Journal of the American Oil Chemists Society

Abstract

A high-speed, high-resolution approach to silvercomplexation chromatography of triacylglycerols in vegetable fats and cocoa butter equivalents is described. Trisaturated and major isomeric unsaturated species with up to five double bonds are eluted within 12 min. A simple change of solvent composition enables elution of species with up to nine double bonds within the same time-frame. Throughput is three samples/hr (70 samples/24 hr with an automatic system). Short-term reproducibility of analytical data for the system gives standard deviations similar to those obtained in practice for high-resolution capillary gas chromatography of triacylglycerols, and quantitative accuracy is good. Details of column preparation, solvent gradient and system operation are given.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. De Vries, B.,Chem. Ind. (London) 1049 (1962).

  2. Barrett C.B., M.S.J. Dallas and F.B. Padley,J. Am. Oil Chem. Soc. 40:580 (1963).

    Article  CAS  Google Scholar 

  3. Dallas M.S.J., and F.B. Padley,Lebensm.-Wiss. u. Technol. 10: 328 (1977).

    CAS  Google Scholar 

  4. Hammond E.W.,Chemistry Ind. (London) 20:110 (1981).

    Google Scholar 

  5. Smith E.C., A.D. Jones and E.W. Hammond,J. Chrom. 188:205 (1980).

    Article  CAS  Google Scholar 

  6. Aitzetmuller K.,Prog. Lipid Res. 21:111 (1982).

    Google Scholar 

  7. Hammond, E.W., and J.W. Irwin,HPLC in Food Analysis, edited by R. Macrae, Academic Press Ltd., Academic Press Ltd., 1988, pp. 96-132.

  8. Tkkano S., and Y. Kondoh,J. Am. Oil Chem. Soc. 64:380 (1987).

    Article  Google Scholar 

  9. Christie, W.W.,J. High Res. Chrom, and Chrom. Comm. 10:148 (1987).

    Article  CAS  Google Scholar 

  10. Christie, W.W.,J. Chrom. 454:213 (1988).

    Article  Google Scholar 

  11. Litchfield, C,Analysis of Triglycerides, Academic Press, 1972, pp. 248-250.

  12. Geeraert, E., and P. Sandra,J. High. Res. Chrom, and Chrom. Comm. 8:415 (1985).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unilever Research Laboratory, Colworth House, MK44 1LQ, Sharnbrook, Bedford, UK

    B. S. J. Jeffrey

Authors
  1. B. S. J. Jeffrey
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Jeffrey, B.S.J. Silver-complexation liquid chromatography for fast, high-resolution separations of triacylglycerols. J Am Oil Chem Soc 68, 289–293 (1991). https://doi.org/10.1007/BF02657678

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02657678

Key words

Search

Navigation