Abstract
Methylation of canola oil deodorizer distillate catalyzed by a nonspecific lipase was investigated. The conversion of fatty acids to methyl esters has been optimized by using a statistical design. Up to 96.5% conversion of fatty acids to their methyl esters has been achieved without the aid of vacuum or any water-removing agent. The effects of temperature, ratio of the reactants (methanol: fatty acids in the deodorizer distillate) and enzyme concentration on the equilibrium conversion were studied. The temperature and ratio of the reactants showed a significant effect on the conversion of fatty acids to methyl esters and they exhibited a strong interactive effect. Enzyme concentration in the range of 2.7% to 4.3% did not show a significant effect on the equilibrium conversion of fatty acids. Greater than 95% conversion of fatty acids to methyl esters was achieved at temperatures around 50°C and at a ratio of the reactants between 1.8 and 2.0. The inhibitory effect of hydrophilic methanol on the enzyme activity was largely reduced by working at the lower temperature range (around 50°C).
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References
Charles, M.,J. Am. Oil Chem. Soc. 65:1936 (1988).
Sampath, K., U.S. patent 4,594,437 (1986).
Wilging, S.M., and R.R. Swanson, European patent 171,009 (1986).
Shishikura, A., K. Fujimoto, T. Kaneda, K. Arai and S. Saito,Yukagaku 37:8 (1988).
Wilging, S.M., U.S. patent 4,550,183 (1985).
Ishii, K., S. Asano, N. Sashita, T. Kanada, and S. Ito., Japanese patent 60,185,776 (1985).
Sebrell Jr., W.H., and R.S. Harris, inVitamins, Vol. 5, 2nd edn., Academic Press, New York, NY, 1972, p. 166.
Iwai, M., Y. Tsujisaka and K. Fujimoto,J. Gen. Appl. Microbiol. 10:13 (1964).
Ibrahim, C.O., N. Nishio and S. Nagai,Agric. Biol. Chem. 51:2153 (1987).
Knez, Z., M. Ieitgeb., D. Zavnsnk, and B. Lavric,Fat Sci. Technol. 92:169 (1990).
Okumura, S., M. Iwai and Y. Tsujisaka,Biochim. Biophys. Acta 575:156 (1979).
Welsh, F.W., W.D. Murray and R.E. Williams,Crit. Rev. in Biotechnol. 9:138 (1989).
Box, G.E.P., and N.R. Draper, inEmpirical Model-Building and Response Surfaces, John Wiley & Sons, Inc., New York, NY, 1987.
Khuri, A.I., and J.A. Cornell, inResponse Surfaces-Designs and Analyses, Marcel Dekker, Inc., New York, NY, 1987.
AOCS Official and Tentative Methods, edited by David Firestone, Vol. 2, 3rd edn., American Oil Chemists' Society, Champaign, IL, 1989, Method Te 1a-64.
Ibid., Vol. 1, Method Cd 3b-76.
Ibid., Vol. 2, Method Tg 1a-64.
Ibid., Vol. 1, Method Ca 6b-53.
Deming, N.S., and S.L. Morgan, inData Handling in Science and Technology, Experimental Design. A Chemometric Approach, Vol. 3, Elsevier, New York, NY, 1987, p. 149.
Golden Software, Inc.,Surfer, Version 4, Colorado, 1989.
Welsh, F.W., and R.E. Williams,Enzyme Microb. Technol. 2:743 (1990).
Novo Laboratories Ltd.,Sp-382-Preliminary Product Information, Novo Alle', DK-2880 Bagsvaerd, Denmark, 1989.
Svanholm, H., Enzymatic Interesterification and Esterification, paper presented at the AOCS short course on speciality fats, Kings Island, OH, 1989.
Klibanow, A.M.,ChemTech 16:354 (1986).
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Ramamurthi, S., Bhirud, P.R. & McCurdy, A.R. Enzymatic methylation of canola oil deodorizer distillate. J Am Oil Chem Soc 68, 970–975 (1991). https://doi.org/10.1007/BF02657545
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DOI: https://doi.org/10.1007/BF02657545