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Induction, detection and characterization of cell differentiation mutants inDrosophila

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Summary

Mitotic recombination has been used as a tool for detecting cell differentiation mutants in clones of heterozygous individuals. With this method, previously mutagenized (ethyl methanesulfonate) genomes can be screened for mutants, induced anywhere in the genome, in a first generation, and irrespectively of being lethal themselves or present ina lethal chromosome. Induced mitotic recombination was used again in order to give the chromo-some arm location and recombinational locus.

In this way 13 new cell differentiation mutants out of 1504 studied genomes have been detected, meiotically mapped and isolated. They affect the process of cell differentiation of chaetes and/or trichomes in the adult cuticle of the thorax and/or the tergites.

A mitotic chromosome map has been constructed using the mitotic recombination data of these new mutants and those of other cell differentiation mutants already available.

The applicability of this method for the detection of other cell mutants in other systems is discussed.

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References

  • Beadle, G. W., Tatum, E. L.:Neurospora II. Methods of producing and detecting mutations concerned with nutritional requirements. Amer. J. Bot.32, 678–686 (1945)

    Article  Google Scholar 

  • Becker, A. J.: The influence of he~rochromatin inversion heterozygosity and somatic pairing on X-ray induced mitotic recombination inDrosophila mclanogaster, Molec. gen. Genet.105, 203–218 (1969)

    Article  PubMed  CAS  Google Scholar 

  • Benzer, S.: Behavioral mutants ofDrosophila isolated by countercurrent distribution. Proc. nat. Acad. Sci. (Wash.)58, 1112–1119 (1967)

    Article  Google Scholar 

  • Demerec, M.: Biological action of small defienciencies of the X-chromosome ofDrosophila melanogaster. Proc. nat. Acad. Sci. (Wash.)20, 354–359 (1934)

    Article  CAS  Google Scholar 

  • Epstein, R. H., Bolle, A., Steinberg, C. M., Kellenberger, E., Boy de la Tour, E., Chevalley, R., Edgar, R. S., Susman, M., Denhardt, G. H., Lielausis, A.: Physiological studies of con-ditional lethal mutants of bacteriophage T4D. Cold Spr. Harb. Symp. quant. Biol.28, 375–394 (1963)

    CAS  Google Scholar 

  • Garcia-Bellido, A.: Some parameters of mitotic recombination inDrosophila melanogaster. Molec. gen. Genet.115, 54–72 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Bellido, A.: The corrected number of adult epidermal cells of the tergites. Drosophila Inform. Serv. (in press)

  • Garcia-Bellido, A., Merriam, J. R.: Parameters of the wing imaginal disc development ofDrosophila melanogaster. Develop. Biol.24, 61–87 (1971a)

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Bellido, A., Merriam, J. R.: Clonal parameters of tergite development inDrosophila. Develop. Biol.26, 264–276 (1971b)

    Article  PubMed  CAS  Google Scholar 

  • I-Ialvorson, H. O.: Sequential expression of biochemical events during intracellular differentia-tion. Syrup. Soc. Gen. Microbiol.15, 343–368 (1965)

    Google Scholar 

  • Judd, B. H., Shen, H. W., Kaufman, J. C.: The anatomy and function of a segment of the X chromosome ofDrosophila melanogaster. Genetics71, 139–156 (1972)

    PubMed  CAS  Google Scholar 

  • Lewis, E. B., Bacher, F.: Method for feeding ethyl methane sulphonate (EMS) toDrosophila males. Drosophila Inform. Serv.48, 193 (1968)

    Google Scholar 

  • Lifschytz, E., Falk, R.: Fine structure analysis of a chromosome segment inDrosophila melanogaster. Analysis of ethyl methane sulphonate-induced lethals. Mutation Res.8, 147–155 (1969)

    PubMed  CAS  Google Scholar 

  • Lindsley, D. L., Grelt, E. H.: Genetic variations inDrosophila melanogaster. Carnegie Inst. Wash. PubL No 627 (1968)

  • Merriam, J. R., Garcia-Bellido, A.: A model for somatic crossing over with third chromosome rearrangements inDrosophila melanogaster. Molec. gem Genet.115, 302–313 (1972)

    Article  CAS  Google Scholar 

  • Merriam, J. R., Nothiger, R., Garcia-Bellido, A.: Are dicentric anaphase bridges formed by somatic recombination in X chromosome inversion heterozygotes ofDrosophila melano-gaster ? Molec. gen. Genet.115, 294–301 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Ripoll, P., Garcia-Bellido, A.: Cell autonomous lethals inDrosophila melanogaster. Nature (Lond.) New Biol.241, 15–16 (1973)

    CAS  Google Scholar 

  • Sandler, L., Lindsley, D. L., Nicoletti, B., Trippa, G.: Mutants affecting meiosis in natural populations ofDrosophila melanogaster. Genetics60, 525–528 (1968)

    PubMed  CAS  Google Scholar 

  • Stern, C.: Somatic crossing over and segregation inDrosophila melanogaster. Genetics21, 625–730 (1936)

    PubMed  CAS  Google Scholar 

  • Stern, C.: Genetic mosaics and other essays. Cambridge, Mass: Harvard Univ. Press 1968

    Google Scholar 

  • Suzuki, D. T., Piterniek, L. K., Hayashi, S., Tarasoff, M., Baillie, D., Erasmus, U.: Tempe-rature-sensitive mutations inDrosophila melanogaster, I. Relative frequencies among X-ray and chemically induced sex-linked recessive lethals and semiletha]s. Proc. nat. Aead. Sei. (Wash.)57, 907–912 (1967)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Centro de Investigaciones Biologieas C.S.I.C., Instituto de Genetica y Antropologia, Madrid, Spain

    A. Garcia-Bellido & J. Dapena

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  1. A. Garcia-Bellido
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  2. J. Dapena
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Communicated by E. Itadorn.

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Garcia-Bellido, A., Dapena, J. Induction, detection and characterization of cell differentiation mutants inDrosophila . Molec. Gen. Genet. 128, 117–130 (1974). https://doi.org/10.1007/BF02654485

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  • DOI: https://doi.org/10.1007/BF02654485

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