Summary
The adsorption analysis of six binary mixtures composed of ethyl stearate, ethyl oleate, ethyl linoleate, and ethyl linolenate has been studied on alumina columns. Distillation methods have been shown by other workers to fractionate the stearate-oleate system but not the oleate-linoleate system. Application of the adsorption analysis technique resulted in fractionation of all six systems including the oleate-linoleate mixture.
Measures of the performance of the columns were obtained by dividing the eluate into two portions at the point in the fractionation at which the starting composition was reached. The first component to be eluted from each of the six pairs was obtained in the first fraction with an average purity of between 68 and 83%. The second component was obtained in the second fraction with average purity of 61 to 76%. The first component to be eluted was obtained in higher purity than the second. The efficiency of separation of pairs appeared to depend on the difference in degree of unsaturation of the components. Overall recoveries of esters from the columns ranged from 71.8 to 87.5%.
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One of the laboratories of the Bureau of Agricultural and Industrial Chemistry, Agricultural Research Administration, U. S. Department of Agriculture.
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Dutton, H.J., Reinbold, C.L. Adsorption analysis of lipids. III. Synthetic mixtures of ethyl stearate, oleate, linoleate, and linolenate. J Am Oil Chem Soc 25, 120–124 (1948). https://doi.org/10.1007/BF02637646
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DOI: https://doi.org/10.1007/BF02637646