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Factors affecting resistance of low density lipoproteins to oxidation

  • Antioxidant Status and Peroxidation
  • Published:
Lipids

Abstract

Oxidation resistance (OR) of low density lipoproteins (LDL) is frequently determined by the conjugated diene (CD) assay, in which isolated LDL is exposed to Cu2+ as prooxidant in the range of 1–10 μM. A brief review on major findings obtained with this assay will be given. A consistent observation is that vitamin E supplements or oleic acid-rich diets increase OR. Oxidation indices measured by the CD assay and effects of antioxidants very significantly depend on the Cu2+ concentration used for LDL oxidation. For medium and high Cu2+ concentrations, the relationship between lag time and propagation rate can be described by a simple hyperbolic saturation function, which has the same mathematical form as the Michaelis-Menten equation. At medium and high Cu2+ concentrations (0.5 to 5 μM), vitamin E increases lag time in a dose-dependent manner. The increase is higher for 0.5 μM Cu2+ as compared to 5 μM. At low Cu2+ concentrations (0.5 μM or less), the mechanism of LDL oxidation changes. Significant oxidation occurs in a preoxidation phase, which commences shortly after addition of Cu2+. Preoxidation is not inhibited by vitamin E. It is concluded that much additional work is needed to validate the importance of oxidation indices derived from CD and similar assays.

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Abbreviations

CD:

conjugated diene

LDL:

low density lipoprotein

OR:

oxidation resistance

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Authors and Affiliations

  1. Institute of Biochemistry, University of Graz, Schubertstraße 1, A-8010, Graz, Austria

    O. Ziouzenkova, S. P. Gieseg, P. Ramos & H. Esterbauer

Authors
  1. O. Ziouzenkova
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  2. S. P. Gieseg
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  3. P. Ramos
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  4. H. Esterbauer
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Ziouzenkova, O., Gieseg, S.P., Ramos, P. et al. Factors affecting resistance of low density lipoproteins to oxidation. Lipids 31, S71–S76 (1996). https://doi.org/10.1007/BF02637054

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  • DOI: https://doi.org/10.1007/BF02637054

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