Abstract
Incremental pressure programming was demonstrated to be an effective technique for increasing the yield of 90% pure allcis-5,8,11,14,17-ethyl eicosapentaenoate (EPA) in the fractionation of urea-crystallized fish oil ethyl esters using supercritical fluid CO2. The fractionations, which also produced high purity allcis-4,7,10,13,16,19-ethyl docosahexaenoate (DHA), were performed using a column temperature gradient. In initial experiments, the maximum temperature of the superimposed gradient was 80°C, and processing pressures ranged from 1900–2200 psi. By reduction of processing pressures, comparable yields of EPA were obtained from fractionations in which the maximum temperatures in the gradient were 70°C and 60°C.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Leaf, A. and P.C. Weber,N. Eng. J. Med. 318:549 (1988).
Kremer, J.M., A.V. Michalek, L. Lininger, C. Huyck, J. Bigauoette, M.A. Timchalk, R.I. Rynes, J. Zieminski and L.E. Bartholomew,Lancet 1 (8422):184 (1985).
Lands, W.E.M.,Fish and Human Health, Academic Press, Inc., Orlando, FL, 1986.
Kinsella, J.E.,Seafoods and Fish Oils in Human Health and Disease, Marcel Dekker, Inc., NY, 1987.
Simopoulos, A.P.,J. Nutr. 116:2350 (1986).
Nilsson, W.B., V.F. Stout and J.K. Hudson,J. Am. Oil Chem. 63:470, Abstract 353P (1986).
Nilsson, W.B., E.J. Gauglitz, Jr., J.K. Hudson, V.F. Stout and J. Spinelli,J. Am. Oil Chem. Soc. 65:109 (1988).
Nilsson, W.B., E.J. Gauglitz, Jr., J.K. Hudson, and F.M. Teeny, Presented at the 194th American Chemical Society Meeting, New Orleans, LA, September 1987.
Eisenbach, W.,Ber. Bunsenges. Phys. Chem. 88:882 (1984).
McHugh, M.A., and V.J. Krukonis,Supercritical Fluid Extraction Principles and Practice, Butterworths, Boston, MA, 1986, p. 191.
Rizvi, S.S.H., R.R. Chao and Y.J. Liaw, inSupercritical Fluid Extraction and Chromatography, ACS Symposium Series 366, edited by B.A. Charpentier and M.R. Sevenants, American Chemical Society, Washington, DC, 1988, p. 89.
Stout, V.F., and J. Spinelli, U.S. Patent 4,675,132 (1987).
Krukonis, V.J., C.J. Bambara, J.E. Vivian, W.B. Nilsson and R.E. Martin, Presented at the 194th American Chemical Society Meeting, New Orleans, LA, September 1987.
Krukonis, V.J., inSupercritical Fluid Extraction and Chromatography, ACS Symposium Series 366, edited by B.A. Charpentier and M.R. Sevenants, American Chemical Society, Washington, DC, 1988, p. 26.
Domart, C., D.T. Miyauchi and W.N. Sumerwell,J. Am. Oil Chem. Soc. 32:481 (1955).
Brule, M.R., and R.W. Corbett,Hydrocarbon Processing 63(6):73 (1984).
Debenedetti, P.G., and S.K. Kumar,AIChE J. 34:645 (1988).
Klesper, E., and F.P. Schmitz,J. Supercrit. Fluids 1:45 (1988).
McHugh, M.A., and V.J. Krukonis,Supercritical Fluid Extraction Principles and Practice, Butterworths, Boston, 1986, pp. 153–157.
Zosel, K.,Angew. Chem. Int. Ed. Engl. 17:702 (1978).
Gauglitz, E.J., Jr. and L.W. Lehman,J. Am. Oil Chem. Soc. 40:197 (1963).
Prange, M.M., and W.H. Riepe,Chem. Eng. Process. 22:183 (1987).
Paulautis, M.E., V.J. Krukonis, R.T. Kurnik and R.C. Reid,Rev. Chem. Eng. 1:179 (1983).
Lee, M.L., and K.E. Markides,Science 235:1342 (1987).
Author information
Authors and Affiliations
About this article
Cite this article
Nilsson, W.B., Gauglitz, E.J. & Hudson, J.K. Supercritical fluid fractionation of fish oil esters using incremental pressure programming and a temperature gradient. J Am Oil Chem Soc 66, 1596–1600 (1989). https://doi.org/10.1007/BF02636184
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02636184