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Transforming growth factor-beta: Its effect on phenotype reexpression by dedifferentiated chondrocytes in the presence and absence of osteogenin

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Summary

The single and combined actions of transforming growth factor (TGF)-beta and osteogenin were evaluated with regard to induction of colony formation and reexpression of the differentiated phenotype by dedifferentiated rabbit articular chondrocytes in soft agarose under serum-free conditions. TGF-beta alone did not promote colony formation and induced accumulation of proteoglycans and type II collagen at significantly lower levels than those induced by osteogenin. Although synergism between these two growth factors occurred with respect to the induction of colony formation, their joint action on reexpression of the differentiated phenotype was additive. Complex interactions between the two growth factors may explain the latter phenomenon.

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Authors and Affiliations

  1. Bone Cell Biology Section, Laboratory of Cellular Development and Oncology, National Institute of Dental Research, National Institutes of Health, 20892, Bethesda, Maryland

    Edward T. Harrison Jr., Frank P. Luyten & A. H. Reddi

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  1. Edward T. Harrison Jr.
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  2. Frank P. Luyten
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  3. A. H. Reddi
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Harrison, E.T., Luyten, F.P. & Reddi, A.H. Transforming growth factor-beta: Its effect on phenotype reexpression by dedifferentiated chondrocytes in the presence and absence of osteogenin. In Vitro Cell Dev Biol - Animal 28, 445–448 (1992). https://doi.org/10.1007/BF02634049

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  • DOI: https://doi.org/10.1007/BF02634049

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