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An efficient method for routine epstein-barr virus immortalization of human B lymphocytes

  • Genetics
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Summary

A variety of methods exist for the immortalization of B lymphocytes by Epstein-Barr virus due to the simplicity of such techniques to establish cell lines with stable genomic DNA. Two different methods for immortalizing lymphoblastoid cell lines were compared for differences in techniques and materials, time between initiation and immortalization, and success rate of immortalization. An incubation period in Epstein-Barr virus and the use of conditioned media improved immortalization efficiency from 86 to 98% and decreased the time (usually weeks) from culture initiation to cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in non-insulin-dependent diabetes mellitus.

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Authors and Affiliations

  1. Center for Human Cell Biotechnology, Department of Surgery, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284-7842, San Antonio, Texas

    Florence E. Wall & Mary Pat Moyer

  2. Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas

    Richard D. Henkel

  3. Department of Medicine, The University of Texas Health Science Center at San Antonio, 78284, Texas

    Michael P. Stern

  4. Department of Pediatrics, The University of Texas Health Science Center at San Antonio, 78284, Texas

    Hal B. Jenson

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  5. Mary Pat Moyer
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Wall, F.E., Henkel, R.D., Stern, M.P. et al. An efficient method for routine epstein-barr virus immortalization of human B lymphocytes. In Vitro Cell Dev Biol - Animal 31, 156–159 (1995). https://doi.org/10.1007/BF02633976

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  • DOI: https://doi.org/10.1007/BF02633976

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