Summary
Immune responses to live and inactivated avian adenovirus-1 were evaluated in broilers by neutralising antibody response and challenge reaction. The neutralising antibody titre was 1:256, 1:64 and 1:32 in live virus, inactivated virus and uninoculated control birds respectively at 3 weeks post inoculation when they were challenged. At one week post challenge the antibody titres were 1:941, 1:247 and 1:375 in live virus, inactivated virus and control birds, respectively. There was a booster effect of challenge in the live virus inoculated group up to 3 weeks post challenge. The post challenge histopathological evaluation of liver, kidneys, lungs and bursa revealed less severe changes in the live virus inoculated group as compared to other groups.
Résumé
Les réponses immunologiques utilisant des adenovirus aviaires inactivés ou vivants furent évaluées chez des poulets après challenge et utilisation d’anticorps neutralisants. Le titre d’anticorps neutralisants fut respectivement de 1 : 256, 1 : 64 et 1 : 32 pour les oiseaux inoculés avec des virus vivants, des virus inactivés et sans inoculation et ceci 3 semaines inoculation au moment du challenge: une semaine après le challenge le titre fut de 1 : 941, 1 : 247 et 1 : 375 pour les même groupes. Une stimulation immunologique fut observée, 3 semaines après le challenge, pour le groupe inoculé avec des virus vivants. Une étude histopathologique effectuée après le challenge sur le foie et les reins montrèrent des changements moindres chez le groupe inoculé avec des virus vivants que chez les 2 autres groupes.
Resumen
Se evaluaron las respuestas inmunológicas del adenovirus-1 aviar activo e inactivado en broilers con anticuerpos neutralizantes y reacción a la inoculación. El título de anticuerpos neutralizante fue de 1 : 256, 1 : 64 y 1 : 32 en virus activos, virus inactivados y aves control no inoculadas respectivamente a las 3 semanas después de la inoculación, cuando fueron estimulados. A la primera semana después de la estimulación el título de anticuerpos fue de 1 : 941, 1 : 247 y 1 : 375 en virus activos, virus inactivados y broilers control, respectivamente. Hubo un efecto réfuerzo de activación en el grupo de aves inoculadas con el virus activo a partir de la 3 semana después de la inoculación. La evaluación histopatológica después de la inoculación en hígado, riñones, pulmones y bolsa reveló cambios menos graves en el grupo inoculado con virus activos que en los otros grupos comparados.
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Kaur, A., Oberoi, M.S. & Singh, A. Neutralising antibody and challenge response to live and inactivated avian adenovirus-1 in broilers. Trop Anim Health Prod 29, 141–146 (1997). https://doi.org/10.1007/BF02633009
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DOI: https://doi.org/10.1007/BF02633009