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In vitro clonal multiplication of 4-year-old plants of the bamboo,Dendrocalamus longispathus kurz

  • Micropropagation
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Summary

A complete protocol for micropropagation of 4-yr-old plants of the bambooDendrocalamus longispathus is described. Culture initiation was strongly influenced by the nature of the explant and the season. In vitro multiplication was achieved through forced axillary branching. Single node segments from the young lateral branches produced multiple shoots on agar-solidified Murashige and Skoog (MS) medium supplemented with 12µM benzylaminopurine (BAP) and 3µM kinetin. The shoots have been multiplied for 15 passages in liquid and thereafter for over 5 passages on semisolid MS+15µM BAP+1µM indolebutyric acid (IBA)+10% coconut water at a rate of 3.2- and 2.8-fold, every 4 wk, respectively. The nature of the propagule was a critical factor for shoot multiplication and rooting. Seventy-three percent of the shoots rooted on a modified MS medium (major salts reduced to half strength) containing 1µM indoleacetic acid, 1µM IBA, and 68µM coumarin. Through a simple in vitro hardening step, more than 85% of the tissue culture-raised plants were successfully transferred to soil.

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References

  • Banik, R. L. Seed germination of some bamboo species. Indian For. 113:578–586; 1987.

    Google Scholar 

  • Banik, R. L. Technique of bamboo propagation with special reference to pre-rooted and pre-rhizomed branch cuttings and tissue culture. In: Rao, A. N.; Dhanarajan, G.; Sastry, C. B., eds. Recent research on bamboos. Chinese Academy of Forestry, Beijing and IDRC, Singapore: 1987:160–169.

    Google Scholar 

  • Constantine, D. In vitro multiplication of woody species. In: Round Table Conf., Gembloux, Belgium; 1978:134.

  • CSIR. The Wealth of India: A dictionary of Indian raw materials and industrial products: Raw Material-3, New Delhi: CSIR; 1952:32–33.

    Google Scholar 

  • Davis, M. J.; Baker, R.; Hanan, J. J. Clonal multiplication of carnation by micropropagation. J. Am. Soc. Hortic. Sci. 102:48–53; 1977.

    CAS  Google Scholar 

  • Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158; 1968.

    Article  PubMed  CAS  Google Scholar 

  • Hammerschlag, F. A. Factors influencing in vitro multiplication and rooting of the plum rootstock Myrobalan (Prunus cerasifera Ehrh.). J. Am. Soc. Hortic. Sci. 107(1):44–47; 1982a.

    Google Scholar 

  • Hammerschlag, F. A. Factors affecting establishment and growth of peach shoot tips in vitro. Hortscience 17:85–86; 1982b.

    Google Scholar 

  • Huang, L. C.; Huang, B. L.; Chen, W. L. Tissue culture investigations of bamboo—IV. Organogenesis leading to adventitious shoots and plants in excised shoot apices. Environ. Exp. Bot. 19:307–315; 1989.

    Article  Google Scholar 

  • Hyndman, S. E.; Hasegawa, P. M.; Bressan, R. A. Stimulation of root initiation from cultured rose shoots through the use of reduced concentration of mineral salts. Hortscience 17:82–83; 1982.

    CAS  Google Scholar 

  • Kumar, A.; Dhawan, M.; Gupta, B. B. Vegetative propagation of bamboo using growth promoting substances. Ind. For. 114:569–575; 1988.

    Google Scholar 

  • Lane, W. D. Regeneration of pear plants from shoot meristems. Plant Sci. Lett. 16:337–342; 1979.

    Article  CAS  Google Scholar 

  • Monette, P. L. INfluence of size of culture vessel on in vitro proliferation of grape in a liquid medium. Plant Cell Tissue Organ Cult. 2:327–332; 1983.

    Article  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Nadgir, A. L.; Phadke, C. H.; Gupta, P. K., et al. Rapid multiplication of bamboo by the tissue culture. Silvae Genet. 33:219–223; 1984.

    Google Scholar 

  • Prutpongse, P.; Gavinlertvatana, P. In vitro micropropagation of 54 species from 15 genera of bamboo. Hortscience 27:453–454; 1992.

    CAS  Google Scholar 

  • Quoirin, M.; Lepoivre, PH. Improved medium for in vitro culture ofPrunus sp. Acta Hortic. 78:437–442; 1977.

    Google Scholar 

  • Roohi, F. N.; Shamsi, F. Q.; Rao, I. V. R., et al. Clonal propagation of matureBambusa balcooa in vitro. In: Abstracts IV International Bamboo Workshop, Chiangmai, Thailand. Kasetsart University, Bangkok, Royal Forest Department Bangkok and IDRC, Singapore; 1991:71.

    Google Scholar 

  • Ryugo, K.; Breen, P. J. Indoleacetic acid metabolism in cuttings of plum (Prunus cerasifera XP. munsoniana cv. Mariana 2624). Proc. Am. Soc. Hortic. Sci. 99:247; 1974.

    CAS  Google Scholar 

  • Saxena, S. In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation. Plant Cell Rep. 9:431–434; 1990.

    Article  CAS  Google Scholar 

  • Saxena, S.; Bhojwani, S. S. Regeneration and mass propagation of bamboo species through tissue culture. Proc. of IV International Bamboo Workshop, Chiangmai, Thailand, Nov. 24–29, 1991. Kasetsart University, Bangkok, Royal Forest Department Bangkok and IDRC, Singapore; in press.

  • Seethalakshmi, K. K.; Venkatesh, C. S.; Surendran, T. Vegetative propagation of bamboo using growth promoting substances-1.Bambusa balcooa Roxb. Ind. J. For. 2:98–103; 1983.

    Google Scholar 

  • Skirvin, R. M.; Chu, M. C.; Rukan, H. Tissue culture of peach, sweet and sour cherry, and apricot shoot tips. In: Proc. III State Hortic. Soc. 113:30–38; 1980.

    Google Scholar 

  • Sood, A.; Palni, L. M. S.; Sharma, M., et al. Micropropagation ofDendrocalamus hamiltonii Munro using single node cuttings taken from elite seedling plants. In: Abstracts IV International Bamboo Workshop, Chiangmai, Thailand. Kasetsart University, Bangkok, Royal Forest Department, Bangkok and IDRC, Singapore; 1991:65.

    Google Scholar 

  • Surendran, T.; Venkatesh, C. S.; Seethalakshmi, K. K. Vegetative propagation of the thorny bambooBambusa arundinacea (Retz.) Willd. using some growth regulators. J. Tree Sci. 2(1–2):10–15; 1983.

    Google Scholar 

  • Sutter, E.; Langhans, R. W. Epicuticular wax formation on carnation plantlets regenerated from shoot tip culture. J. Am. Soc. Hortic. Sci. 2:327–332; 1979.

    Google Scholar 

  • Tsay, H. S.; Yeh, C. C.; Hsu, J. Y. Embryogenesis and plant regeneration from anther culture of bamboo (Sinocalamus latiflora [Munro] McClure). Plant Cell Rep. 9:349–351; 1990.

    Article  Google Scholar 

  • Yeh, M. L.; Chang, W. C. Plant regeneration through somatic embryogenesis in callus cultures of green bamboo (Bambusa oldhami Munro). Theor. Appl. Genet. 173:161–163; 1986a.

    Article  Google Scholar 

  • Yeh, M. L.; Chang, W. C. Somatic embryogenesis and subsequent plant regeneration from inflorescence cells ofBambusa beecheyana Munro var. beecheyana. Plant Cell Rep. 5:409–411; 1986b.

    Article  Google Scholar 

  • Zamora, A. B.; Grezo, S. S.; Damasco, O. P. Callus induction and plant regeneration from internode tissues ofDendrocalamus latiflorus cv. Machiku. In: Rao, A. N.; Yusoff, A. M., eds. Proceedings of the Seminar Tissue Culture of Forest Species. Forest Research Institute of Malaysia, Kuala Lumpur and IDRC, Singapore; 1989:76–82.

    Google Scholar 

  • Ziv, M. In vitro hardening and acclimatization of tissue culture plants. In: Withers, L. A.; Anderson, P. G., eds. Plant tissue culture and its agricultural applications. London: Butterworths; 1986:187–196.

    Google Scholar 

  • Ziv, M. Enhanced shoot and cormlet proliferation in liquid cultured gladiolus buds by growth retardants. Plant Cell Tissue Organ Cult. 17:101–110; 1989.

    Article  CAS  Google Scholar 

  • Ziv, M.; Schwarts, A.; Fleminger, D. Malfunctioning stomata in vitreous leaves of carnation (Dianthus caryophyllus) plants propagated in vitro; implications for hardening. Plant Sci. 52:127–134; 1987a.

    Article  CAS  Google Scholar 

  • Ziv, M.; Schwarts, A.; Fleminger, D. Hardening aspects of micropropagated carnation plants having malfunctioning stomata. In: Ducate, G.; Jacob, M.; Simeon, A., eds. Plant micropropagation in horticultural industries. Liege, Belgium: Presses Universitaires; 1987b:47–54.

    Google Scholar 

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Authors and Affiliations

  1. Tata Energy Research Institute, 90 Jor Bagh, 110 003, New Delhi

    Sanjay Saxena & Sant S. Bhojwani

  2. Department of Botany, University of Delhi, 110 007, Delhi, India

    Sant S. Bhojwani

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  1. Sanjay Saxena
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  2. Sant S. Bhojwani
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Saxena, S., Bhojwani, S.S. In vitro clonal multiplication of 4-year-old plants of the bamboo,Dendrocalamus longispathus kurz. In Vitro Cell Dev Biol - Plant 29, 135–142 (1993). https://doi.org/10.1007/BF02632285

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  • DOI: https://doi.org/10.1007/BF02632285

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