Abstract
A semimicro method for determining both total fatty acids and unsaponifiable matter in fats, fatty acids, and soap is presented. The procedure involves saponification and recovery of the total fatty acids (which includes the unsaponifiables), removal of the fatty acids with anion exchange resins, and determination of the unsaponifiable matter by weight.
The total fatty acid method was evaluated on seven samples and showed a standard deviation from the mean of 0.21%. The unsaponifiable matter determination gives good reproducibility with a standard deviation from the mean of 0.06%. Since this ion exchange is a direct determination of the nonionic components, the results obtained from it should be more accurate and more nearly the true values than the macro extraction method which is empirical.
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Benedict, J.H. Semimicro determination of total fatty acids and unsaponifiable matter. J Am Oil Chem Soc 37, 415–418 (1960). https://doi.org/10.1007/BF02631197
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DOI: https://doi.org/10.1007/BF02631197