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Isolation and primary cultures of human intrahepatic bile ductular epithelium

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Summary

A technique for the isolation of human intrahepatic bile ductular epithelium, and the establishment of primary cultures using a serum- and growth-factor-supplemented medium combined with a connective tissue substrata is described. Initial cell isolates and monolayer cultures display phenotypic characteristics of biliary epithelial cells (low molecular weight prekeratin positive; albumin, alphafetoprotein, and Factor VIII-related antigen negative). Ultrastructural features of the cultured cells show cell polarization with surface microvilli, numerous interepithelial junctional complexes and cytoplasmic intermediate prekeratin filaments.

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Authors and Affiliations

  1. Department of Pathology, Presbyterian University Hospital, Pittsburgh, PA

    A. J. Demetris, S. Saidman, S. Graner & R. Duquesnoy

  2. Department of Surgery, Presbyterian University Hospital, Pittsburgh, PA

    B. H. Markus, L. Makowka & T. E. Starzl

  3. Department of Surgery, University of Rochester, NY

    J. J. Fung

Authors
  1. A. J. Demetris
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  2. B. H. Markus
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  3. S. Saidman
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  4. J. J. Fung
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  5. L. Makowka
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  6. S. Graner
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  7. R. Duquesnoy
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  8. T. E. Starzl
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Demetris, A.J., Markus, B.H., Saidman, S. et al. Isolation and primary cultures of human intrahepatic bile ductular epithelium. In Vitro Cell Dev Biol 24, 464–470 (1988). https://doi.org/10.1007/BF02628499

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  • DOI: https://doi.org/10.1007/BF02628499

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