Summary
Cells from mechanically dissociated postblastokinetic embryos of the rangeland grasshopper,Melanoplus sanguinipes, were seeded in Falcon Primaria culture dishes and monitored for attachment, growth, and differentiation. Various sonicated nutrient phospholipid combinations, including both natural complexes and specific synthetic lipid types, were tested on these embryonic tissues as supplements in combination with three serum-containing basal media. The combined use of Primaria culture vessels and lipid supplementation supported the growth of various epithelioid as well as fibroblastoid and other cell types. Some cells became tightly associated into partially differentiated tissuelike growths that included networks of ducted tracheolarlike cells, ducted granular epithelioid cells, and networks and sheets of slowly contracting muscle cells. The tissue-associated cell types and two individually growing cell types (a plasmatocytoid or hemocytic cell and a peculiar blak granule-containing cell) were serially subcultured from three to nine times in Primaria vessels. Tissues subcultured in standard plastic culture vessels were selected by this growth surface for fibroblastoid cell types or expressed a fibroblastoid morphology, or both, within 1 to 3 passages. The growth of cells bearing neuritelike and glialike processes was stimulated in the primary cultures by certain medium and lipid combinations, but these cell types were not subcultivable.
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Bettger, W. J.; Boyce, S. T.; Walthall, B. J., et al. Rapid clonal growth and serial passage of human diploid fibroblasts in a lipid-enriched synthetic medium supplemented with epidermal growth factor insulin, and dexamethasone. Proc. Natl. Acad. Sci. USA 78:5588–5592; 1981.
Bissell, M. J.; Hall, H. G.; Parry, G. How does the extra cellular matrix direct gene expression? J. Theor. Biol. 99:31–68; 1982.
Blau, H. M.; Webster, C. Isolation and characterization of human muscle cells. Proc. Natl. Acad. Sci. USA 78:5623–5627; 1981.
Chen, J. S.; Del Fa, A.; Di Luzio, A., et al. Liposome-induced morphological differentiation of murine neuroblastoma. Nature 263:604–606; 1976.
Cherbas, L.; Fristrom, J. W.; O'Connor, J. D. The action of ecdysone in imaginal discs and Kc cells ofDrosophila melanogaster. In: Hoffmann, J. H.; Porchet, M., eds. Biosynthesis, metabolism and mode of action of invertebrate hormones. Berlin: Springer-Verlag; 1984:305–322.
Cheesebeauf, M.; Padieu, P. Rat liver epithelial cell cultures in a serum-free medium: primary cultures and derived cell lines expressing differentiated functions. In Vitro 20:780–795; 1984.
Cripps, C.; de Renobales, M.; Blomquist, G. J. De novo biosynthesis of linoleic acid in insects. Biochim. Biophys. Acta 876:572–580; 1986.
Dadd, R. H. Essential fatty acids: insects and vertebrates compared. In: Mittler, T. E.; Dadd, R. H., eds. Metabolic aspects of lipid nutrition in insects. Boulder, CO: westview Press; 1983:107–147.
Dubendorfer, A.; Eichenberger-Glinz, S. Development and metamorphosis of larval and adult tissues ofDrosophila in vitro. In: Kurstak, E.; Maramorosch, K.; Dubendorfer, A., eds. Invertebrate systems in vitro. Amsterdam: Elsevier/North Holland Biomedical Press; 1980:169–185.
Gelernter, W. D.; Federici, B. A. Continuous cell line fromSpodoptera exigua (Lepidoptera: Noctuidae) that supports replication of nuclear polyhedrosis viruses fromSpodoptera exigua andAutographa californica. J. Invertebr. Pathol. 48:199–207; 1986.
Goodwin, R. H. Growth of insect cells in serum-free medium. In: Techniques in the life sciences, cell biology, vol. C1, Techniques in setting up and maintenance of tissue and cell cultures. County Clare, Ireland: Elsevier Scientific Publishers Ireland Ltd.; 1985; separate C109, 23 pp.
Hasin, Y.; Sapoznikov, D.; Stein, O., et al. Effect of fatty acid composition of rat heart myocytes on their electrical activity. J. Mol. Cell. Cardiol. 14:163–171; 1982.
Kurtti, T. J. Phenotypic variations of cell lines from cockroach embryos. In: Maramorosch, K., ed. Invertebrate tissue culture research applications. New York: Academic Press; 1976:39–56.
Lagarde, M.; Sicard, B.; Guichardant, M., et al. Fatty acid composition in native and cultured human endothelial cells. In Vitro 20:33–37; 1984.
Lynn, D. E.; Feldlaufer, F. Synthesis of the insect steroid hormone 20-hydroxyecdysone by an insect cell line from the cabbage looper. In Vitro 22 (3), Part II; 1986:13A.
Lynn, D. E.; Miller, S. G.; Oberlander, H. Establishment of a cell line from lepidopteran wing imaginal discs: induction of newly synthesized proteins by 20-hydroxyecdysone. Proc. Natl. Acad. Sci. USA 79:2589–2593; 1982.
Oberlander, H.; Lynn, D. E. Morphogenesis in insect tissue culture, In: Maramorosch, K., ed. Advances in cell culture, vol. 2. New York: Academic Press; 1982:237–265.
Rochford, R.; Dougherty, E. M.; Lynn, D. E. Establishment of a cell line from embryos of the cabbage looper,Trichoplusia ni (Hubner). In Vitro 20:823–825; 1984.
Schaeffer, W. I. Usage of vertebrate, invertebrate and plant cell, tissue and organ culture terminology. In Vitro 20:19–24; 1984.
Tsang, K. R.; Freeman, F. A.; Kurtti, T. J., et al. New Cell lines from embryos ofMelanoplus sanguinipes (F.) (Orthoptera: Acrididae). Acrida 10:105–111; 1981.
Ward, G. B., Jr.; Kelly, T. J. Examination of ecdysteroid production and secretion in 10 established insect cell lines. In Vitro 22 (3) Part II: 10; 1986:13A.
Wigglesworth, V. B. The physiology of insect tracheoles. In: Berridge, M. J.; Treherne, J. E.; Wigglesworth, V. B., eds. Advances in insect physiology, vol. 17. London: Academic Press; 1983:85–148.
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Goodwin, R.H. The effect of lipids on the subculture of differentiated cells from primary cultures of grasshopper embryonic tissues. In Vitro Cell Dev Biol 24, 388–400 (1988). https://doi.org/10.1007/BF02628490
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DOI: https://doi.org/10.1007/BF02628490