Summary
Mammary tumor epithelial cells from BALB/cfC3H mice were dispersely embedded inside the collagen gels in Ham's F-12 medium containing horse serum. A sustained cell growth leading to a 5- to 10-fold increase in cell number over initial level was observed in less than 2 weeks. The extent of this growth was found to be dependent on serum concentration. However, addition of various protein and steroid hormones, both singly and in combination, to low-serum-containing medium failed to achieve a comparable level of growth to that promoted by higher serum concentration. Mammary tumor cells can now be consistently propagated in primary culture.
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This investigation was supported by Grants CA05388 and CA09041 awarded by the National Cancer Institute, Department of Health, Education and Welfare, and by cancer research funds of the University of California.
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Yang, J., Guzman, R., Richards, J. et al. Primary culture of mouse mammary tumor epithelial cells embedded in collagen gels. In Vitro 16, 502–506 (1980). https://doi.org/10.1007/BF02626463
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DOI: https://doi.org/10.1007/BF02626463