Skip to main content
SpringerLink
Log in

Natural and induced synchronous cultures

  • Symposium: Cell Cycles and Synchronized Cell Populations
  • Published:
In Vitro Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Howard, A., and S. R. Pelc. 1953. Synthesis of deoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage. Heredity 6: (Suppl.) 261–273.

    CAS  Google Scholar 

  2. Painter, R. B., and R. M. Drew. 1959. Studies on deoxyribonucleic acid metabolism in human cancer cell cultures (HeLa). 1. The temporal relationships of deoxyribonucleic acid synthesis to mitosis and turnover time. Lab. Invest. 8: 278–285.

    PubMed  CAS  Google Scholar 

  3. Lajtha, L. G., R. Oliver, and F. Ellis. 1954. Incorporation of32P and adenine14C into DNA by human bone marrow cells in vitro. Brit. J. Cancer 8: 367–379.

    PubMed  CAS  Google Scholar 

  4. Stanners, C. P., and J. E. Till. 1960. DNA synthesis in individual L-strain mouse cells. Biochim. Biophys. Acta 37: 406–419.

    Article  PubMed  CAS  Google Scholar 

  5. Mitchison, J. M. 1969. Enzyme synthesis in synchronous cultures. Science 165: 657–663.

    Article  PubMed  CAS  Google Scholar 

  6. Till, J. E., G. F. Whitmore, and S. Gulyas. 1963. Deoxyribonucleic acid synthesis in individual L-strain mouse cells. II. Effects of thymidine starvation. Biochim. Biophys. Acta 72: 277–289.

    Article  PubMed  CAS  Google Scholar 

  7. Rao, P. N., and J. Engelberg. 1966. Effects of temperature on the mitotic cycle of normal and synchronized mammalian cells. In: I. L. Cameron and G. M. Padilla (eds.),Cell Synchrony Studies in Biosynthetic Regulation. Academic Press, New York, pp. 332–352.

    Google Scholar 

  8. Engelberg, J., and H. R. Hirsch. 1966. On the theory of synchronous cultures. In: I. L. Cameron and G. M. Padilla (eds.),Cell Synchrony Studies in Biosynthetic Regulation. Academic Press, New York, pp. 14–37.

    Google Scholar 

  9. Petersen, D. F., R. A. Tobey, and E. C. Anderson. 1969. Synchronously dividing mammalian cells. Fed. Proc. 28: 1771–1779.

    PubMed  CAS  Google Scholar 

  10. Thompson, L. H., and H. D. Suit. 1969. Proliferation kinetics of X-irradiated mouse L-cells studied with time-lapse photography. Int. J. Radiat. Biol. 15: 347–362.

    Article  CAS  Google Scholar 

  11. Elkind, M. M., and G. F. Whitmore. 1967.The Radiobiology of Cultured Mammalian Cells. Gordon and Breach, New York.

    Google Scholar 

  12. Burnett-Hall, D. G., and W. A. O'N. Waugh. 1967. Indices of synchrony in cellular cultures. Biometrics 23: 693–716.

    Article  PubMed  CAS  Google Scholar 

  13. Sinclair, R. 1970. Synchrony in cultured animal cells. In vitro 5: 79–93.

    Article  PubMed  CAS  Google Scholar 

  14. Hahn, G. M. 1966. State vector description of the proliferation of mammalian cells in tissue culture. I. Exponential growth. Biophys. J. 6: 275–290.

    PubMed  CAS  Google Scholar 

  15. Hahn, G. M., and R. F. Kallman. 1967. State vector description of the proliferation of mammalian cells in tissue culture. II. Effects of single and repeated exposures to ionizing radiations. Radiat. Res. 30: 702–713.

    Article  PubMed  CAS  Google Scholar 

  16. Tobey, R. A., E. C. Anderson, and D. F. Petersen. 1966. RNA stability and protein synthesis in relation to the division of mammalian cells. Proc. Nat. Acad. Sci. U. S. A. 56: 1520–1527.

    Article  CAS  Google Scholar 

  17. Tobey, R. A., D. F. Petersen, E. C. Anderson, and T. T. Puck. 1966. Life cycle analysis of mammalian cells. III. The inhibition of division in Chinese hamster cells by puromycin and actinomycin. Biophys. J. 6: 567–581.

    PubMed  CAS  Google Scholar 

  18. Fujiwara, Y. 1967. role of RNA synthesis in DNA replication of synchronized populations of cultured mammalian cells. J. Cell. Physiol. 70: 291–300.

    Article  PubMed  CAS  Google Scholar 

  19. Mittermayer, C., P. Kaden, U. Trommershaueser, and W. Sandritter. 1968. Initiation of DNA synthesis in a system of synchronized L cells. Effect of actinomycin D. Histochemie 14: 113–122.

    Article  PubMed  CAS  Google Scholar 

  20. Terasima, T., and M. Yasukawa. 1966. Synthesis of G1 protein preceding DNA synthesis in cultured mammalian cells. Exp. Cell Res. 44: 669–672.

    Article  PubMed  CAS  Google Scholar 

  21. Martin, D. W., G. M. Tomkins, and M. A. Bresler. 1969. Control of specific gene expression examined in synchronized mammalian cells. Proc. Nat. Acad. Sci. U. S. A. 63: 842–849.

    Article  CAS  Google Scholar 

  22. Hodge, L. D., E. Robbins, and M. D. Scharff. 1969. Persistence of messenger RNA through mitosis in HeLa cells. J. Cell Biol. 40: 497–507.

    Article  PubMed  CAS  Google Scholar 

  23. Steward, D. L., J. R. Shaeffer, and R. M. Humphrey. 1968. Breakdown and assembly of polyribosomes in synchronized Chinese hamster cells. Science 161: 791–793.

    Article  PubMed  CAS  Google Scholar 

  24. Robbins, E., and T. W. Brown. 1967. The cytoplasmic synthesis of histones in HeLa cells and its temporal relationship to DNA replication. Proc. Nat. Acad. Sci. U. S. A. 57: 409–416.

    Article  CAS  Google Scholar 

  25. Terasima, T., and L. J. Tolmach. 1963. Variations in several responses of HeLa cells to X-irradiation during the division cycle. Biophys. J. 3: 11–33.

    PubMed  CAS  Google Scholar 

  26. Whitmore, G. F., S. Gulyas, and G. Boland. 1965. Radiation sensitivity throughout the cell cycle and its relationship to recovery. In:Cellular Radiation Biology. The Williams and Wilkins Company, Baltimore, pp. 423–441.

    Google Scholar 

  27. Sinclair, W. K., and R. A. Morton. 1966. X-ray sensitivity during the cell generation cycle of cultured Chinese hamster cells. Radiat. Res. 29: 450–474.

    Article  PubMed  CAS  Google Scholar 

  28. Sinclair, W. K. 1965. Hydroxyurea: differential lethal effects on cultured mammalian cells during the cell cycle. Science 150: 1729–1731.

    Article  PubMed  CAS  Google Scholar 

  29. Elkind, M. M., K. Sakamoto, and C. Kamper. 1968. Age-dependent toxic properties of actinomycin D and X-rays in cultured Chinese hamster cells. Cell Tissue Kinet. 1: 209–224.

    CAS  Google Scholar 

  30. Madoc-Jones, H., and F. Mauro. 1968. Interphase action of vinblastine and vincristine: differences in their lethal action through the mitotic cycle of cultured mammalian cells. J. Cell. Physiol. 72: 186–196.

    Article  Google Scholar 

  31. Bacchetti, S., and G. F. Whitmore. 1969. Actinomycin D: effects on mouse L cells. Biophys. J. 9: 1427–1445.

    PubMed  CAS  Google Scholar 

  32. Bacchetti, S., and G. F. Whitmore. 1969. The action of hydroxyurea on mouse L-cells. Cell Tissue Kinet. 2: 193–211.

    CAS  Google Scholar 

  33. Djordjevic, B., and J. H. Kim. 1967. Lethal effect of phleomycin in different stages of the division cycle of HeLa cells. Cancer Res. 27: 2255–2260.

    PubMed  CAS  Google Scholar 

  34. Barski, G., S. Sorieul, and F. Cornefert. 1961. “Hybrid” type cells in combined cultures of two different mammalian cell strains. J. Nat. Cancer Inst. 26: 1269–1277.

    PubMed  CAS  Google Scholar 

  35. Klebe, R. J., I. Chen, and F. H. Ruddle. 1970. Controlled production of proliferating somatic cell hybrids. J. Cell Biol. 45: 74–82.

    Article  PubMed  CAS  Google Scholar 

  36. Roosa, R. A., and E. Bailey. 1970. DNA-mediated transformation of mammalian cells in culture. Increased transforming efficiency following sonication. J. Cell. Physiol. 75: 137–150.

    Article  PubMed  CAS  Google Scholar 

  37. Campbell, A. 1957. Synchronization of cell division. Bacteriol. Rev. 21: 263–272.

    PubMed  CAS  Google Scholar 

  38. Swann, M. M. 1957. The control of cell division: A review. I. General mechanisms. Cancer Res. 17: 727–757.

    PubMed  CAS  Google Scholar 

  39. Wildy, P., and A. A. Newton. 1958. The “Synchronous” division of HeLa cells. Biochem. J. 68, 14p.

    Google Scholar 

  40. Newton, A. A., and P. Wildy. 1959. Parasynchronous division of HeLa cells. Exp. Cell Res. 16: 624–635.

    Article  PubMed  CAS  Google Scholar 

  41. Newton, A. A. 1964. Synchronous division of animal cells in culture. In: E. Zeuthen (ed.),Synchrony in Cell Division and Growth. Interscience, New York, pp 441–466.

    Google Scholar 

  42. Miura, T., and T. Vtakoji 1961. Studies on synchronous division of FL cells by chilling. Exp. Cell Res. 23: 452–459.

    Article  Google Scholar 

  43. Stubblefield, E., and G. C. Mueller. 1965. Thymidine kinase activity in synchronized HeLa cell cultures. Biochem. Biophys. Res. Commun. 20: 535–538.

    Article  PubMed  CAS  Google Scholar 

  44. Friedman, D. L., and G. C. Mueller. 1968. A nuclear system for DNA replication from synchronized HeLa cells. Biochim. Biophys. Acta 161: 455–468.

    PubMed  CAS  Google Scholar 

  45. Rueckert, R. R., and G. C. Mueller. 1960. Studies on the unbalanced growth in tissue culture. I. Induction and consequences of thymidine deficiency. Cancer Res. 20: 1584–1591.

    PubMed  CAS  Google Scholar 

  46. Littlefield, J. W. 1962. DNA synthesis in partially synchronized L cells. Exp. Cell Res. 26: 318–326.

    Article  PubMed  CAS  Google Scholar 

  47. Priest, J. H., J. Heady, and R. E. Priest. 1967. Synchronization of human diploid cells by FUdR. The first ten minutes of synthesis in female cells. J. Nat. Cancer Inst. 38: 61–72.

    PubMed  CAS  Google Scholar 

  48. Davies, L. M., J. H. Priest, and R. E. Priest. 1968. Collagen synthesis by cells synchronously replicating DNA. Science 159: 91–93.

    Article  PubMed  CAS  Google Scholar 

  49. Chu, E. H. Y. 1965. Effects of ultra-violet radiation on mammalian cells. II. Differential UV and X-ray sensitivity of chromosomes to breakage in 5-aminouracil synchronized cell population. Genetics 52: 1279–1294.

    PubMed  CAS  Google Scholar 

  50. Galavazi, G., H. Schenk, and D. Bootsma. 1966. Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. I. Optimal conditions. Exp. Cell Res. 41: 428–437.

    PubMed  CAS  Google Scholar 

  51. Xeros, N. 1962. Deoxyriboside control and synchronization of mitosis. Nature (London) 194: 682–683.

    Article  CAS  Google Scholar 

  52. Bootsma, D., L. Budke, and O. Vos. 1964. Studies on synchronous division of tissue culture cells initiated by excess thymidine. Exp. Cell Res. 33: 301–309.

    Article  PubMed  CAS  Google Scholar 

  53. Galavazi, G., and Bootsma, D. 1966. Synchronization of mammalian cellsin vitro by inhibition of DNA synthesis. II. Population dynamics. Exp. Cell Res. 41: 438–451.

    PubMed  CAS  Google Scholar 

  54. Mueller, G. C. 1963. Molecular events in replication of nuclei. Exp. Cell Res. 9: 144–149.

    Article  Google Scholar 

  55. Puck, T. T. 1964. Phasing mitotic delay and chromosomal aberrations in mammalian cells. Science 144: 565–566.

    Article  PubMed  Google Scholar 

  56. Petersen, D. F., and E. C. Anderson. 1964. Quantity production of synchronized mammalian cells in tissue culture. Nature (London) 203: 642–643.

    Article  CAS  Google Scholar 

  57. Doida, Y., and S. Okada. 1967. Synchronization of L5178 Y cells by successive treatment with excess thymidine and colcemid. Exp. Cell Res. 48: 540–548.

    Article  CAS  Google Scholar 

  58. Holmberg, B. 1968. A blockage in the S-phase of HeLa cells produced by a human tumour polypeptide. Europ. J. Cancer 4: 475–480.

    CAS  Google Scholar 

  59. Klevecz, R. R., and E. Stubblefield. 1967. RNA synthesis in relation to DNA replication in synchronized Chinese hamster cell cultures. J. Exp. Zool. 165: 259–268.

    Article  PubMed  CAS  Google Scholar 

  60. Stubblefield, E., and S. Murphee. 1967. Synchronized mammalian cell cultures. II. Thymidine kinase activity in colcemid synchronized fibroblasts. Exp. Cell Res. 48: 652–656.

    Article  CAS  Google Scholar 

  61. Stubblefield, E., R. R., Klevecz, and L. Deaven. Synchronized mammalian cell cultures: cell replication cycle and macromolecular synthesis following brief colcemid arrest of mitosis. J. Cell. Physiol. 69: 345–353.

  62. Kim, J. H. and B. K. Stambuk. 1966. Synchronization of HeLa cells by vinblastine sulphate. Exp. Cell Res. 44: 631–634.

    Article  PubMed  CAS  Google Scholar 

  63. Pfeiffer, S. E., and L. J. Tolmach. 1967. Selecting synchronous population of mammalian cells. Nature (London) 213: 139–142.

    Article  CAS  Google Scholar 

  64. Brinklev, B. R., E. Stubblefield, and T. C. Hsu. The effect of colcemid inhibition and reversal on the fine structure of the mitotic apparatus of Chinese hamster cells in vitro. J. Ultrastruct. Res. 19: 1–18.

  65. Cohen, L. S., and G. P. Studzinski. 1967. Correlation between cell enlargement and nucleic acid and protein content of HeLa cells in unbalanced growth produced by inhibitors of DNA synthesis. J. Cell. Physiol. 69: 331–340.

    Article  PubMed  CAS  Google Scholar 

  66. Whitmore, G. F., J. E. Till, and S. Gulyas. 1967. Radiation-induced mitotic delay in L cells. Radiat. Res. 30: 155–171.

    Article  PubMed  CAS  Google Scholar 

  67. Pfeiffer, S. E., and L. J. Tolmach. 1967. Inhibition of DNA synthesis in HeLa cells by hydroxyurea. Cancer Res. 27: 124–129.

    PubMed  CAS  Google Scholar 

  68. Whitmore, G. F., and S. Gulyas. 1966. Synchronization of mammalian cells with tritiated thymidine. Science 151: 691–694.

    Article  PubMed  CAS  Google Scholar 

  69. Becker, A. J., E. A. McCulloch, L. Siminovitch, and J. E. Till. 1965. The effect of differing demands for blood cell production on DNA synthesis by hemopoietic colony-forming cells of mice. Blood 26: 296–308.

    PubMed  CAS  Google Scholar 

  70. Till, J. E., and E. A. McCulloch. 1961. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat. Res. 14: 213–222.

    Article  PubMed  CAS  Google Scholar 

  71. Axelrad, A. A., and E. A. McCulloch. 1958. Obtaining suspensions of animal cells in metaphase from cultures propagated on glass. Stain Techn. 33: 67–71.

    PubMed  CAS  Google Scholar 

  72. Terasima, T., and L. J. Tolmach. 1963. Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp. Cell Res. 30: 344–362.

    Article  PubMed  CAS  Google Scholar 

  73. Lindall, P. 1966. A new method for the continuous selection of cells in mitosis. Exp. Cell Res. 43: 424–434.

    Article  Google Scholar 

  74. Robbins, E., and M. D. Scharff. 1967. The absence of a detectable G1 phase in a cultured strain of Chinese hamster lung cell. J. Cell Biol. 34: 684–686.

    Article  PubMed  CAS  Google Scholar 

  75. Tobey, R. A., E. C. Anderson, and D. F. Petersen. 1967. Properties of mitotic cells prepared by mechanically shaking monolayer cultures of Chinese hamster cells. J. Cell. Physiol. 70: 63–68.

    Article  PubMed  CAS  Google Scholar 

  76. Gonzales, Sister P., and R. M. Nardone. 1968. Cyclical nucleolar changes during the cell cycle. 1. Variations in number, size, morphology, position. Exp. Cell Res. 50: 599–615.

    Article  Google Scholar 

  77. Brent, T. P., K. A. V. Butler, and A. R. Crathorn. 1965. Variations in phosphokinase activities during the cell cycle in synchronous populations of HeLa cells. Nature (London) 207: 176–177.

    Article  CAS  Google Scholar 

  78. Griffin, M. J., and R. Ber. 1969. Cell cycle events in the hydrocortisone regulation of alkaline phosphatase in HeLa S3 cells. J. Cell Biol. 40: 297–304.

    Article  PubMed  CAS  Google Scholar 

  79. Mittermayer, C., P. Kaden, and W. Sandritter. 1968. Studies of synchronously dividing L-cells. 1. Optimal growth conditions and characterization of synchronous cell systems. Histochemie 12: 67–74.

    Article  PubMed  CAS  Google Scholar 

  80. King D., and M. Barnhisel. 1967. Synthesis of RNA in mammalian cells during mitosis and interphase. J. Cell Biol. 33: 265–272.

    Article  PubMed  CAS  Google Scholar 

  81. Sinclair, W. K., and R. A. Morton. 1963. Variations in X-ray responses during the division cycle of partially synchronized Chinese hamster cells in culture. Nature (London) 199: 1158–1160.

    Article  CAS  Google Scholar 

  82. Sinclair, R., and D. H. L. Bishop. 1965. Synchronous culture of strain L mouse cells. Nature (London) 205: 1272–1273.

    Article  Google Scholar 

  83. Morris, N. R., J. W. Cramer, and D. Reno. 1967. A simple method for concentration of cells in the DNA synthetic period of the mitotic cycle. Exp. Cell Res. 48: 216–218.

    Article  PubMed  CAS  Google Scholar 

  84. Miller, R. G., and R. A. Phillips. 1969. Separation of cells by velocity sedimentation. J. Cell. Physiol. 73: 191–202.

    Article  PubMed  CAS  Google Scholar 

  85. MacDonald, H. R., and R. G. Miller. Synchronization of mouse L cells by a velocity sedimentation technique. Biophys. J. in press.

  86. Puck, T. T., and J. Steffan. 1963. Life cycle analysis of mammalian cells. 1. A method for localizing metabolic events within the life cycle and its applications to the action of colcemid and sublethal doses of X-irradiation. Biophys. J. 3: 379–397.

    Article  PubMed  CAS  Google Scholar 

  87. Petersen, D. F., R. A. Tobey, and E. C. Anderson. 1969. Biosynthetic activity in synchronized cells. In: G. M. Padilla, G. L. Whitson and I. L. Cameron (eds.),The Cell Cycle: Gene-Enzyme Interactions. Academic Press, New York, pp. 341–359.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Canada

    G. F. Whitmore

Authors
  1. G. F. Whitmore
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Whitmore, G.F. Natural and induced synchronous cultures. In Vitro 6, 276–285 (1971). https://doi.org/10.1007/BF02625942

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02625942

Keywords

Search

Navigation