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Cultivation of HeLa cells with fetal bovine serum or ultroser G: Effects on the plasma membrane constitution

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Summary

Plasma membranes isolated from HeLa cells cultivated in suspension cultures supplemented with 3.5% fetal bovine serum or 2% of the commercially available serum substitute Ultroser G contained the same amounts of protein, cholesterol, and phosphate on a cellular basis. Minor differences in the plasma membrane fatty acid composition were seen, with the most pronounced alteration observed for palmitic acid, which amounted to 27 and 20% in fetal bovine serum- and Ultroser G-supplemented cells, respectively. Plasma membranes from cells growth with Ultroser G contained almost twice as much phosphatidylethanolamine and displayed two thirds of the phosphatidylcholine content, compared to plasma membranes obtained from fetal bovine serum supplemented cells. The former membranes also showed a 3 times higher specific [3H]acetate labeling of cholesterol, indicating a higherde novo synthesis of cholesterol. Both quantitative and qualitative alterations were revealed among the plasma membrane polypeptides when these were subjected to immuno- and lectin blottings. Fluorescence anisotropy measurements at different temperatures produced similar results irrespective of the growth medium supplement when the plasma membrane specific probe 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene was used on intact cells. However, the average cellular rigidity was higher for Ultroser G supplemented cells, determined with 1,6-diphenyl-1,3,5-hexatriene as a probe.

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Authors and Affiliations

  1. Department of Microbiology, University of Lund, Sölvegatan 21, S-223 62, Lund, Sweden

    Ylva Blixt & Einar Everitt

  2. Laboratory of Ecological Chemistry, Ecology Building, Helgonavägen 5, S-223 62, Lund, Sweden

    Anders Valeur

Authors
  1. Ylva Blixt
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  2. Anders Valeur
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  3. Einar Everitt
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Additional information

This investigation was supported by grants from the Swedish Natural Science Research Council, Anders Otto Swärds Stiftelse, Stockholm, Crafoordska Stiftelsen, Lund and Kungl. Fysiografiska Sällskapet, Lund.

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Blixt, Y., Valeur, A. & Everitt, E. Cultivation of HeLa cells with fetal bovine serum or ultroser G: Effects on the plasma membrane constitution. In Vitro Cell Dev Biol 26, 691–700 (1990). https://doi.org/10.1007/BF02624425

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  • DOI: https://doi.org/10.1007/BF02624425

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