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Non-morphogenic metabolism during shoot induction in radiata pine cotyledon explants

  • Plant Cellular and Developmental Biology
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Summary

The effects of excision, light and cytokinin (N6-benzyladenine) on14C-acetate metabolism in cotyledons ofPinus radiata (D. Don) were determined.14CO2 was released and the distribution of radioactivity into lipids, sugars, organic acids and amino acids was determined. While light and cytokinin generally caused some increase in metabolism, the effect of excision, i.e., wounding, was most pronounced. Specific metabolites examined (citrate, malate, succinate, alanine, aspartate, glutamate and glutamine) were at least 50% greater in14C-labeling in excised cotyledons as compared to intact seedlings. This enhancement of wound metabolism would mask possible morphogenically-related changes occurring at that time.

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References

  1. Aitken, J.; Horgan, K. J.; Thorpe, T. A. Influence of explant selection on the shoot-forming capacity of juvenile tissue ofPinus radiata. Can. J. For. Res. 11:112–117; 1981.

    Google Scholar 

  2. Asahi, T.; Honda, Y.; Uritani, I. Increase of mitochondrial fraction in sweet potato root tissue after wounding or infection withCeratocystis fimbriata. Plant Physiol. 41:1179–1184; 1986.

    Article  Google Scholar 

  3. Baraldi, R.; Rossi, F.; Lercari, B. In vitro shoot development ofPrunus GF 655-2: interaction between light and benzyladenine. Physiol. Plant. 74:440–443; 1988.

    Article  CAS  Google Scholar 

  4. Bender, L.; Joy, IV, R. W.; Thorpe, T. A. Studies on [14C]-glucose metabolism during shoot bud induction in cultured cotyledon explants ofPinus radiata. Physiol. Plant. 69:428–434; 1987.

    CAS  Google Scholar 

  5. Bender, L.; Joy, IV, R. W.; Thorpe, T. A. [14C]-glucose metabolism during shoot bud development in cultured cotyledon explants ofPinus radiata. Plant Cell Physiol. 28:1335–1338; 1987.

    CAS  Google Scholar 

  6. Brenner, M.; Niederweiser, A.; Pataki, G. Thin layer chromatography of amino acids and derivatives. In: Stahl, E., ed. Thin layer chromatography, 2nd ed. New York: Springer-Verlag. 1969:739–751.

    Google Scholar 

  7. Douglas, T. J.; Villalobos, V. M.; Thompson, M. R., et al. Lipid and pigment changes during shoot initiation in cultured explants ofPinus radiata. Physiol. Plant. 55:470–477; 1982.

    Article  CAS  Google Scholar 

  8. Feige, B.; Gimmler, H.; Jeschke, W. D., et al. Eine methode zur dünnschichtchromatographischen auftrennung von14C- und32P-markierten stoffwechselprodukten. J. Chromatogr. 41:80–90; 1969.

    Article  PubMed  CAS  Google Scholar 

  9. Goodwin, T. W.; Mercer, E. I. Introduction to plant biochemistry, 2nd ed. London: Pergamon Press; 1988.

    Google Scholar 

  10. Kahl, G. Metabolism in plant storage tissue slices. Bot. Rev. 40:263–314; 1974.

    CAS  Google Scholar 

  11. Kar, M.; Feierlund, J. Changes in the activities of enzymes involved in amino acid metabolism during the senescence of detached wheat leaves. Physiol. Plant. 63:39–44; 1984.

    Article  Google Scholar 

  12. Kumar, P. P.; Bender, L.; Thorpe, T. A. Activities of ribulose bisphosphate carboxylase and phosphoenol pyruvate carboxylase and14C-bicarbonate fixation duringin vitro culture ofPinus radiata cotyledons. Plant Physiol. 87:675–679; 1988.

    PubMed  CAS  Google Scholar 

  13. Lercari, B.; Tognoni, F.; Anselmo, G., et al. Photocontrol of in vitro bud differentiation inSaintpaulia ionantha leaves andLycopersicon esculentum cytoledons. Physiol. Plant. 67:340–344; 1986.

    Article  CAS  Google Scholar 

  14. Obata-Sasamoto, H.; Beaudoin-Eagan, L.; Thorpe, T. A.14C-metabolism during callus induction in tobacco. Plant Cell Tiss. Org. Cult. 3:291–299; 1984.

    Article  CAS  Google Scholar 

  15. Thorpe, T. A.; Meier, D. D. Starch metabolism in shoot-forming tobacco callus. J. Exp. Bot. 25:288–294; 1974.

    Article  CAS  Google Scholar 

  16. Uritani, I; Asahi, T. Respiration and related activity in wounded and infected tissues. In: Stumpf, P. K.; Conn, E. E., eds. The biochemistry of plants, vol. 2. Academic Press, Inc. 1980:463–485.

  17. Villalobos, V. M.; Leung, D. W. M; Thorpe, T. A. Light-cytokinin interaction in shoot formation in cultured cotyledon explants of radiata pine. Physiol. Plant. 61:497–504; 1984.

    Article  CAS  Google Scholar 

  18. Villalobos, V. M.; Oliver, M. J.; Yeung, E. C., et al. Cytokinininduced switch in development in excised cotyledons of radiata pine cultured in vitro. Physiol. Plant. 61:483–489; 1984.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, T2N 1N4, Calgary, Alberta, Canada

    Richard W. Joyiv & Trevor A. Thorpe

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  1. Richard W. Joyiv
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  2. Trevor A. Thorpe
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This research was supported by the Natural Sciences and Engineering Research Council of Canada Grant A-6467 to T.A. Thorpe.

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Joyiv, R.W., Thorpe, T.A. Non-morphogenic metabolism during shoot induction in radiata pine cotyledon explants. In Vitro Cell Dev Biol 26, 643–646 (1990). https://doi.org/10.1007/BF02624215

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  • DOI: https://doi.org/10.1007/BF02624215

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