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Effects of flow on the synthesis and release of fibronectin by endothelial cells

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Summary

Human umbilical vein endothelial cells at confluence were subjected to steady shear flow. The effect of flow on the synthesis of fibronectin, its release into the medium, and incorporation into the extracellular matrix were investigated. The total content of fibronectin in endothelial cells exposed to flow was found to be lower than that in static controls after periods of 12 to 48 h. In the presence of cycloheximide there was no difference in the fibronectin content of sheared and unsheared cells. Our results suggest that the synthesis of fibronectin is inhibited by the flow-induced perturbation of endothelial cells.

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References

  1. Ando, J.; Komatsuda, J.; Kamiya, A. Cytoplasmic calcium response to fluid shear stress in cultured vascular endothelial cells. In Vitro Cell. Dev. Biol. 24(9):871–877; 1988.

    PubMed  CAS  Google Scholar 

  2. Beckerle, M. Identification of a new protein localized at sites of cell-substrate adhesion. J. Cell. Biol. 103:1679–1687; 1986.

    Article  PubMed  CAS  Google Scholar 

  3. Bhagyalakshmi, A.; Frangos, J. A. Mechanism of shear-induced prostacyclin production in endothelial cells. Biochem. Biophys. Research Comm. 158:31–37; 1989.

    Article  CAS  Google Scholar 

  4. Burridge, K.; Mangeat, P. An interaction between vinculin and talin. Nature 308:744–746; 1984.

    Article  PubMed  CAS  Google Scholar 

  5. de Groot, P. G.; Reinders, J. H.; Sixma, J. J. Perturbation of human endothelial cells by thrombin or PMA changes the reactivity of their extracellular matrix towards platelets. J. Cell Biol. 104:697–704; 1987.

    Article  PubMed  Google Scholar 

  6. Diamond, S. L.; Eskin, S. G.; McIntire, L. V. Laminar shear stress stimulates tissue plasminogen activator secretion by human endothelial cells in culture. Circulation 78(4):510; 1988.

    Google Scholar 

  7. Frangos, J. A.; Eskin, S. G.; McIntire, L. V., et al. Flow effects on prostacyclin production in human endothelial cells. Science 227:1477–1479; 1985.

    Article  PubMed  CAS  Google Scholar 

  8. Frangos, J. A.; McIntire, L. V.; Eskin, S. G. Shear stress induced stimulation of mammalian cell metabolism. Biotechnol. Bioeng. 32:1053–1060; 1988.

    Article  CAS  Google Scholar 

  9. Franke, R. P.; Grafe, M.; Schnittler, H., et al. Induction of human vascular endothelial stress fibres by fluid shear stress. Nature 307:648–649; 1984.

    Article  PubMed  CAS  Google Scholar 

  10. Hallam, T. J.; Pearson, J. D.; Needham, L. A. Thrombin-stimulated elevation of human endothelial cell cytoplasmic free calcium concentration causes prostacyclin production. Biochem. J. 251:243–249; 1988.

    PubMed  CAS  Google Scholar 

  11. Horwitz, A.; Duggan, K.; Buck, C., et al. Interaction of plasma membrane fibronectin receptor with talin-a transmembrane linkage. Nature 320:531–533; 1986.

    Article  PubMed  CAS  Google Scholar 

  12. Kim, D. W.; Langille, B. L.; Wong, M. K. K., et al. Patterns of endothelial microfilament distribution in the rabbit aorta in situ. Circ. Res. 64:21–31; 1989.

    PubMed  CAS  Google Scholar 

  13. Loskutoff, D. J.; Edington, T. S. Synthesis of a fibrinolytic activator and inhibitor by endothelial cells. Proc. Natl. Acad. Sci. USA 74:3903–3907; 1977.

    Article  PubMed  CAS  Google Scholar 

  14. Nawroth, P. P.; Stern, D. M.; Kaplan, K. L., et al. Prostacyclin synthesis by bovine endothelial cells in culture. Blood 64:801–806; 1984.

    PubMed  CAS  Google Scholar 

  15. Pytela, R.; Pierschbacher, M. D.; Ruoslahti, E. Identification and isolation of a 140 kD cell surface glycoprotein with properties expected of a fibronectin receptor. Cell 40:191–198; 1985.

    Article  PubMed  CAS  Google Scholar 

  16. Reinders, J. H.; de Groot, P. G.; Dawes, J., et al. Comparison of secretion and subcellular localization of von Willebrand factor with that of thrombospondin and fibronectin in cultured human vascular endothelial cells. Biochim. Biophys. Acta. 844:306–313; 1985.

    Article  PubMed  CAS  Google Scholar 

  17. Rubanyi, G. M.; Romero, J. C.; Vanhoutte, P. M. Flow induced release of endothelium-derived relaxant factor. Am. J. Physiol. 250:H1145-H1149; 1986.

    PubMed  CAS  Google Scholar 

  18. Ruoslahti, E.; Engvall, E.; Hayman, E. G. Fibronectin: current concepts of its structure and functions. Collagen Res. 1:95–128; 1981.

    CAS  Google Scholar 

  19. Ruoslahti, E.; Hayman, E. G.; Pierschbacher, M. D. Extracellular matrices and cell adhesion. Arteriosclerosis 5:581–594; 1985.

    PubMed  CAS  Google Scholar 

  20. Seidel, C. L.; LaRochelle, J. Venous and arterial endothelia: different dilator abilities in dog vessels. Circ. Res. 60:626–630; 1987.

    PubMed  CAS  Google Scholar 

  21. Tokunaga, O.; Watanabe, T. Properties of endothelial cell and smooth muscle cell cultured in ambient pressure. In Vitro Cell. Dev. Biol. 23(8):528–534; 1987.

    Article  PubMed  CAS  Google Scholar 

  22. Van Wachem, P. B.; Vreriks, C. M.; Beugeling, T., et al. The influence of protein adsorption on interactions of cultured human endothelial cells with polymers. Biomed. Mat. Res. 21:701–718; 1987.

    Article  Google Scholar 

  23. White, G. E.; Gimbrone, M. A.; Fujiwara, K. Factors influencing the expression of stress fibers in vascular endothelial cells. J. Cell Biol. 97:416–424; 1983.

    Article  PubMed  CAS  Google Scholar 

  24. White, G. E.; Fujiwara, K. Expression and intracellular distribution of stress fibers in aortic endothelium. J. Cell Biol. 103:63–70; 1986.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, The Pennsylvania State University, 16802, University Park, Pennsylvania

    Anagha Gupte & John A. Frangos

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  1. Anagha Gupte
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  2. John A. Frangos
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Additional information

This work was supported by grant EET 8708692 from the National Science Foundation, Washington, DC; grant HL-40696 from the National Institutes of Health, Bethesda, MD; and a Research Initiation Grant from the Pennsylvania State University.

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Gupte, A., Frangos, J.A. Effects of flow on the synthesis and release of fibronectin by endothelial cells. In Vitro Cell Dev Biol 26, 57–60 (1990). https://doi.org/10.1007/BF02624155

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