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Physiological and biochemical effects of the mycotoxin patulin on chang liver cell cultures

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Summary

Patulin exhibits both cytotoxic and cytopathic effects on cultured Chang liver cells. The LD50 found was 1.85 μg per ml of patulin. Effects on growth were observed with as little as 0.1 μg per ml of patulin; a 50% reduction in growth was observed at 0.38 μg per ml of patulin. Using a challenge dose of 2.5 μg per ml of patulin, the cytotoxic effect was reversible after an exposure of 10 min, but was not reversible after 20 min. Protein synthesis was depressed after 60 min and RNA synthesis after 20 min of contact with patulin. Neither protein nor RNA synthesis was completely inhibited after 260 min.

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Author information

Author notes

  1. Nancy E. Smith

    Present address: Medical School, Rutgers University, New Brunswick, N.J.

  2. Patricia A. Payne

    Present address: Department of Physiology, Michigan State University, East Lansing, Mich

  3. David M. Wilson

    Present address: Department of Plant Pathology, Univeristy of Georgia Experiment Stations, Tifton, Ga

Authors and Affiliations

  1. College of Medicine, Department of Medical Microbiology, University of Vermont, Given Medical Building, 05401, Vermont, Burlington

    Warren I. Schaeffer, Nancy E. Smith & Patricia A. Payne

  2. Department of Botany, University of Vermont, Marsh Life Sciences Building, 05401, Burlington, Vermont

    David M. Wilson

Authors
  1. Warren I. Schaeffer
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  2. Nancy E. Smith
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  3. Patricia A. Payne
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  4. David M. Wilson
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Additional information

This project was supported in part by NEM-33 Regional Research Project, Cooperative States Research Service.

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Schaeffer, W.I., Smith, N.E., Payne, P.A. et al. Physiological and biochemical effects of the mycotoxin patulin on chang liver cell cultures. In Vitro 11, 69–77 (1975). https://doi.org/10.1007/BF02624078

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  • DOI: https://doi.org/10.1007/BF02624078

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