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Regulation of glucocorticoid receptors and Na−K ATPase activity by hydrocortisone in proximal tubular epithelial cells

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Summary

The effect of hydrocortisone (HC) in modulating glucocorticoid receptors (GR) and sodium-potassium adenosine triphosphatase (Na−K ATPase) activity was studied in primary cultures of immunoisolated murine proximal tubular epithelial cells (PTEC). Utilizing monoclonal antibody against stage-specific embryonic antigen-1, a homogeneous population of PTEC was obtained in high yield. The cells were cultured to confluence and further treated for 48 h in serum-free growth medium containing no HC (control); 50 nM HC; or 50 nM HC plus 20 nM of the antiglucocorticoid, RU 38486. PTEC treated with 50 nM HC had 56% of GR binding and 160% Na−K ATPase activity as compared to controls (P<0.01). GR binding was abolished by incubation in RU 38486 whereas Na−K ATPase fell below control values (P<0.05). Brief incubations of HC-treated PTEC with 0.5 mM ouabain resulted in a fall in GR binding without a change in Na−K ATPase activity. These data indicate that in PTEC, HC regulates GR binding and they suggest that stimulation of Na−K ATPase activity is a direct biological response to this receptor-hormone interaction. Thus, primary cultures of immunoaffinity-isolated PTEC offer a good model system for investigating the molecular basis underlying the regulation of GR binding and postreceptor events influenced by glucocorticoids.

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Authors and Affiliations

  1. Nephrology laboratory, Children's Hospital of Pittsburgh, One Children's Place, 3705 Fifth Avenue at DeSoto Street, 15213, Pittsburgh, PA

    Demetrius Ellis, Tran Dang Sothi, Norman P. Curthoys, Byron Ballou & Ellis D. Avner

  2. Department of Pediatrics, University of Pittsburgh School of Medicine, 15213-3417, Pittsburgh, Pennsylvania

    Demetrius Ellis, Tran Dang Sothi, Norman P. Curthoys, Byron Ballou & Ellis D. Avner

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  1. Demetrius Ellis
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  2. Tran Dang Sothi
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  3. Norman P. Curthoys
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  4. Byron Ballou
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  5. Ellis D. Avner
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Ellis, D., Sothi, T.D., Curthoys, N.P. et al. Regulation of glucocorticoid receptors and Na−K ATPase activity by hydrocortisone in proximal tubular epithelial cells. In Vitro Cell Dev Biol 24, 811–816 (1988). https://doi.org/10.1007/BF02623652

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  • DOI: https://doi.org/10.1007/BF02623652

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