Summary
The stroma vascular fraction of adipose tissue consists of a heterogeneous cell population; not all the cells in this compartment undergo adipose conversion in primary culture. A density gradient centrifugation procedure was used to separate cultured cells on the basis of their triglyceride content. This method was applied to both stroma vascular cells from rat adipose tissue and to a 3T3 F442A preadipose cell line as a reference. Comparison of the results obtained from these two cell types suggests that this separation procedure can lead to a quantification of adipose differentiation in the heterogeneous stroma cell population. Separation procedures were applied to cultured stromal cells derived from young rats during the onset of nutritional obesity induced by overfeeding in early life. Results show that early overfeeding induced an increase in the stromal cell differentiation capacity which is expressed in vitro.
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This work was supported in part by Institut National de la Santé et de la Recherche Médicale (CRL no 82-70-22).
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Dugail, I., Quignard-Boulange, A., Ardouin, B. et al. A method for separating cultured preadipocytes according to their density: Application to stromal cells from overfed suckling rats. In Vitro Cell Dev Biol 22, 375–380 (1986). https://doi.org/10.1007/BF02623525
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DOI: https://doi.org/10.1007/BF02623525