Summary
A hormonally defined medium consisting of a 1∶1 mixture of Dulbecco's modified Eagle's medium and nutrient mixture F12 supplemented with 50 μg/ml each of insulin and transferrin was found to grow late passage malignantly transformed cells of the RL-PR-C rat liver cell culture. The same medium formulation would not grow the early passage, normal, diploid counterpart of the RL-PR-C cell culture. When mixtures of the early and late passage cells were made, only the late passage cells would grow, thus providing a selection system for the late passage cells.
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This work was supported by Grant PHS SO7 05429-20-3 from the National Institutes of Health, Bethesda, MD.
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Schaeffer, W.I., von Kreuter, B.F., Reichard, E.A. et al. The selection of malignantly transformed rat liver cells using a hormonally defined medium. In Vitro 19, 108–110 (1983). https://doi.org/10.1007/BF02621893
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DOI: https://doi.org/10.1007/BF02621893