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Production of IGF-II-related peptide by an anaplastic cells line (AT-3) established from the dunning prostatic carcinoma of rats

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Editor's statement One or more members of the insulin-like growth factor family have been established previously as mitogen for isolated prostate cells. This report suggests that IGF-II member of the family may be involved in autocrine support of cells from highly malignant prostate tumors.

Summary

AT-3 cells, one of anaplastic cell lines established from the Dunning prostatic carcinoma of rats, were able to grow under serum-free conditions in a state of suspension detached from a substratum. Radioimmunoassays using monoclonal antibody against rat insulin-like growth factor II (IGF-II) revealed the presence of IGF-II-related peptide in acid-ethanol extracts extracsts of lyophilized serum-free media conditioned by AT-3 cell. The peptide contents in the culture media increased with increase in cell number; 71 ng at 3.0 × 106 cells and 449 ng at 4.6 × 107 cells. IGF-II-related peptide was hardly detectable in acid-ethanol extracts of AT-3 cells harvested after 13-days culture. These results indicate that AT-3 cells produce IGF-II-related peptide ana may release it into the culture media.

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Authors and Affiliations

  1. Department of Endocrinology, Kagawa Medical School, 1750, Miki-cho, Kita-gun, 761-07, Kagawa, Japan

    Yuhsi Matuo, Nozomu Nishi & Fumio Wada

  2. Amano Pharmaceutical Co. Ltd, 51, Nishiharu-cho, Nishikasugai-gui, 481, Aichi, Japan

    Hideyuki Tanaka & Ikuharu Sasaki

  3. Department of Urology and Oncology Center, School of Medicine, Johns Hopkins University, 21205, Baltimore, Maryland

    John T. Issacs

Authors
  1. Yuhsi Matuo
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  2. Nozomu Nishi
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  3. Hideyuki Tanaka
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  4. Ikuharu Sasaki
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  5. John T. Issacs
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  6. Fumio Wada
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Matuo, Y., Nishi, N., Tanaka, H. et al. Production of IGF-II-related peptide by an anaplastic cells line (AT-3) established from the dunning prostatic carcinoma of rats. In Vitro Cell Dev Biol 24, 1053–1056 (1988). https://doi.org/10.1007/BF02620881

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