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Establishment, characterization, and response to cytotoxic and radiation treatment of three human melanoma cell lines

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Summary

Three human melanoma cell lines were derived from tumor specimens and established in culture. CAL 1 originated from a bone marrow metastasis and CAL 4 and CAL 7 were derived from solid tumor fragments. CAL 1 and CAL 7 were cloned before establishment. Ultrastructural and chromosome analysis were carried out along with the response to nine chemotherapeutic agents at various concentrations. Survival curves after irradiation were also plotted. The uncloned cell line, CAL 4, displayed some differences from the other two cell lines as regards ploidy and response to chemotherapy. Greater spread of chromosome numbers were observed with this cell line, which contained both hypoploid and a hyperploid modal numbers. All three cell lines showed a relatively high extrapolation number after irradiation, suggesting that inherent cellular properties may be partly responsible for the clinical radioresistance of malignant melanomas.

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References

  1. Jensen, O. M.; Bolander, A. M. Trends in malignant melanoma of the skin. World Health Statistics 33: 2–26; 1980.

    CAS  Google Scholar 

  2. Aubert, C.; Rougé, F.; Galindo, J. R. Tumorigenicity of human malignant melanocytes in nude mice in relation to their differentiation in vitro. J. Natl. Cancer Inst. 64: 1029–1040; 1980.

    PubMed  CAS  Google Scholar 

  3. Chen, T. R.; Shaw, M. W. Stable chromsome changes in a human malignant melanoma. Cancer Res. 33: 2042–2047; 1973.

    PubMed  CAS  Google Scholar 

  4. Chen, T. R. Evolution in vitro of stem lines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277–284; 1978.

    PubMed  CAS  Google Scholar 

  5. Giovanella, B. C.; Stehlin, J. S.; Santamaria, C.; Yim, S. O.; Morgan, A. C.; Williams, L. J.; Leibovitz, A.; Fialkow, P. J.; Mumford, D. M. Human neoplastic and normal cells in tissue culture. I. Cell lines derived from malignant melanomas and normal melanocytes. J. Natl. Cancer Inst. 56: 1131–1142; 1976.

    Google Scholar 

  6. Kanzki, T.; Hashimoto, K.; Bath, D. W. Hetero-transplantation of human malignant melanoma cell lines in athymic nude mice. J. Natl. Cancer Inst. 62: 1151–1158; 1979.

    Google Scholar 

  7. Nishihara, T.; Kuriya, Y.; Hayashi, Y.; Ishii, H.; Kimura, M.; Tan, M.; Sasaki, T.; Kasai, M.; Katoh, T.; Matsuya, Y.; Yamane, I. On a new continuous cell line (TM-1) derived from malignant melanoma. Tohoku J. Exp. Med. 128: 217–232; 1979.

    Article  Google Scholar 

  8. Oboshi, S.; Sugano, H. In vitro culture of human cancer cells. Tokyo: Asukara Shoten; 1975.

    Google Scholar 

  9. Romsdahl, M. M.; Hsu, T. C. Establishment and biologic properties of human malignant melanoma cell lines grown in vitro. Surg. Forum 18: 78–79; 1967.

    Google Scholar 

  10. Tveit, K. K.; Fodstad, O.; Johannessen, J. V.; Olsnes, S. A human melanoma cell line established from xenograft in athymic mice. Br. J. Cancer 41: 724–733; 1980.

    PubMed  CAS  Google Scholar 

  11. Whitehead, R. H. Tissue culture studies on human melanoma. Pigment cell. Proceedings of the 8th international pigment cell conference. Vol. 1. Basel: S. Karger AG; 1973: 382–389.

    Google Scholar 

  12. Semple, T. U.; Moore, G. E.; Morgan, R. T.; Woods, L. K.; Quinn, L. A. Multiple cell lines from patients with malignant melanoma: morphology, karyology, and biochemical analysis. J. Natl. Cancer Inst. 68: 365–380; 1982.

    PubMed  CAS  Google Scholar 

  13. Marx, J. L. Tumors: a mixed bag of cells. Science 215: 275–277; 1982.

    Article  PubMed  CAS  Google Scholar 

  14. Salmon, S. E.; Hamburger, A. W.; Soehnlen, B.; Durie, B. G. M.; Alberts, D. S.; Moon, T. E. Quantitation of differential sensitivity of human tumor stem cells to anticancer drugs. N. Engl. J. Med. 298: 1321–1327; 1978.

    Article  PubMed  CAS  Google Scholar 

  15. Dutrillaux, B.; Lejeune, J. Sur une nouvelle technique d’analyse du caryotype humain. C. R. Acad. Sc. Paris 272: 2638–2642; 1971.

    CAS  Google Scholar 

  16. Volm, M.; Kaufman, M.; Hinderer, H.; Goertler, J. Schnellmethode zur sensibilitätstestung malignen tumoren. Klin. Wochenschr. 48: 374–376; 1970.

    Article  PubMed  CAS  Google Scholar 

  17. Alberts, D. S.; Chen, H. S. G.; Salmon, S. E. Tabular summary of pharmacokinetic parameters relevant to in vitro drug assay. Salmon, S. ed. Cloning of human tumor stem cells. New York: A. Liss Inc.; 1980: 351–359.

    Google Scholar 

  18. Weichselbaum, R. R.; Nove, J.; Little, J. B. X-ray sensitivity of human tumor cells in vitro. Int. J. Radiat. Oncol. Biol. Phys. 6: 437–440; 1980.

    PubMed  CAS  Google Scholar 

  19. Bergh, J.; Nilsson, K.; Zech, L.; Giovanella, B. Etablishment and characterization of a continuous lung squamous cell carcinoma cell line (U-1752). Anticancer Res. 1: 317–322; 1981.

    PubMed  CAS  Google Scholar 

  20. Loo, T. L.; Householder, G. E.; Gerulath, A. H.; Sanders, P. H.; Farquhar, D. Mechanism of action and pharmacology studies with DTIC (NSC-45388). Cancer Treat. Rep. 60: 149–152; 1976.

    PubMed  CAS  Google Scholar 

  21. Tveit, K. M.; Fodstad, O.; Olnes, S.; Pihl, A. In vitro sensitivity of human melanoma xenografts to cytotoxic drugs, correlation with in vivo chemosensitivity. Int. J. Cancer. 26: 717–722; 1980.

    Article  PubMed  CAS  Google Scholar 

  22. Meyskens, F. L.; Moon, T. E.; Dana, B.; Gilmartin, E.; Casey, W. J.; G-Chen, H. S.; Hood Franks, D.; Young, L.; Salmon, S. E. Quantitation of drug sensitivity by human metastatic melanoma colony-forming-units. Br. J. Cancer 44: 787–797; 1981.

    PubMed  CAS  Google Scholar 

  23. Mordoh, J.; Chacon, R. D.; Filmus, J. Isolation of tumor cells from patients with osteosarcoma and analysis of their sensitivity to methotrexate. Cancer Res. 41: 3621–3628; 1981.

    PubMed  CAS  Google Scholar 

  24. Hakansson, L.; Trope, C. On the presence within tumors of clones that differ in sensitivity to cytostatic drugs. Acta Pathol. Microbiol. Scand. [A] 82: 35–40; 1974.

    CAS  Google Scholar 

  25. Heppner, G. H.; Dexter, D. L.; De Nucci, T.; Millar, F. R.; Calabresi, P. Heterogeneity in drug sensitivity among tumor cell subpopulations of a single mammary tumor. Cancer Res. 38: 3758–3763; 1978.

    PubMed  CAS  Google Scholar 

  26. Tsuruo, T.; Fidler, I. J. Differences in drug sensitivity among tumor cells from parental tumors, selected variants, and spontaneous metastases. Cancer Res. 41: 3058–3064; 1981.

    PubMed  CAS  Google Scholar 

  27. Guichard, M.; Courdi, A.; Malaise, E. P. Données expérimentales de la radiobiologie des tumeurs solides. J. Eur. Radiother. 1: 171–191; 1980.

    Google Scholar 

  28. Rofstad, E. K. Radiation response of the cells of a human malignant melanoma xenograft. Effect of hypoxic cell radiosensitizers. Radiat. Res. 87: 670–683; 1981.

    Article  PubMed  CAS  Google Scholar 

  29. Selby, P. J.; Courtenay, V. D. In vitro cellular radiosensitivity of human malignant melanoma. Int. J. Radiat. Oncol. Biol. Phys. 8: 1235–1237; 1982.

    PubMed  CAS  Google Scholar 

  30. Fertil, B.; Malaise, E. P. Inherent cellular radiosensitivity as a basic concept for human tumor radiotherapy. Int. J. Radiat. Oncol. Biol. Phys. 7: 621–630; 1981.

    PubMed  CAS  Google Scholar 

  31. Barranco, S. C.; Ramsdahl, M. M.; Humphrey, R. M. The radiation response of human malignant melanoma cells grown in vitro. Cancer Res. 31: 830–833; 1971.

    PubMed  CAS  Google Scholar 

  32. Weininger, J.; Guichard, M.; Joly, A. M.; Malaise, E. P.; Lachet, B. Radiosensitivity and growth parameters in vitro of three human melanoma cell strans. Int. J. Radiat. Biol. 34: 285–290; 1978.

    CAS  Google Scholar 

  33. Trott, K. R.; von Lieven, H.; Kummermehr, J.; Skopal, D.; Lukacs, S.; Braun-Falco, O. The radiosensitivity of malignant melanomas. Part 1: Experimental studies. Int. J. Radiat. Oncol. Biol. Phys. 7: 9–13; 1981.

    PubMed  CAS  Google Scholar 

  34. Guichard, M.; Gosse, C.; Malaise, E. P. Survival curve of a human melanoma in nude mice. J. Natl. Cancer Inst. 58: 1165–1609; 1977.

    Google Scholar 

  35. Chavaudra, N.; Guichard, M.; Malaise, E. P. Hypoxic fraction and repair of potentially lethal radiation damage in two human melanomas transplanted into nude mice. Radiat. Res. 88: 56–68; 1981.

    Article  PubMed  CAS  Google Scholar 

  36. Weichselbaum, R. R.; Schmit, A.; Little, J. B. Cellular repair factors influencing radiocurability of human malignant tumours. Br. J. Cancer 45: 10–16; 1982.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Centre Antoine Lacassagne, 36 Voie Romaine, 06054, Nice Cedex, France

    A. Courdi, J. Gioanni, C. M. Lalanne, J. L. Fischel, M. Schneider & F. Ettore

  2. Faculty of Medicine, Histology Laboratory, Nice University, 06054, Nice Cedex, France

    J. C. Lambert

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  1. A. Courdi
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  2. J. Gioanni
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  3. C. M. Lalanne
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  4. J. L. Fischel
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  5. M. Schneider
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  6. F. Ettore
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  7. J. C. Lambert
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Courdi, A., Gioanni, J., Lalanne, C.M. et al. Establishment, characterization, and response to cytotoxic and radiation treatment of three human melanoma cell lines. In Vitro 19, 453–461 (1983). https://doi.org/10.1007/BF02619592

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  • DOI: https://doi.org/10.1007/BF02619592

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