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Ultrastructural and biological characterization of human choroid cell cultures transformed by Simian Virus 40

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Summary

A human diploid cell line of choroid origin was isolated from the retrouveal portion of an enucleated eye and designated HC. After 10 passages, when the proliferative capacity of HC cells decreased, they were infected and transformed by Simian Virus 40 (SV40). A proliferating long-term cultured cell line designated HC/SV40 was established and it has been maintained as monolayer for more than 100 passages so far. The two cell lines, HC and HC/SV40, were compared for growth characteristics, capacity to form colonies in soft agar, presence of nuclear T-antigen, and ultrastructure. Cytogenetic analysis was also performed to determine the presence of chromosomal aberrations due to the permanent viral transformation of the cell line.

The results indicate that HC/SV40 should be considered the transformed counterparts of HC cells because they are morphologically similar to the latter but can grow in soft agar, possess T-antigen, and show a pattern of karyotypic changes similar to that induced by SV40 in human fibroblasts. The choroid origin of HC and HC/SV40 cell lines was confirmed by the presence, in their cytoplasm, of typical electron dense granules. Their neural origin will make these cell lines very useful for neuropharmacological and differentiation studies.

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References

  1. Hayflick, L.; Moorhead, P. S. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585–621; 1961.

    Article  Google Scholar 

  2. Borek, C. X-Ray-induced in vitro neoplastic transformation of human diploid cells. Nature 283: 776–778; 1980.

    Article  PubMed  CAS  Google Scholar 

  3. Kakunaga, T. Neoplastic transformation of human diploid fibroblast cells by chemical carcinogens. Proc. Natl. Acad. Sci. USA 75: 1334–1338; 1978.

    Article  PubMed  CAS  Google Scholar 

  4. Koprowski, H.; Ponten, J. A.; Jensen, F.; Ravdin, R. G.; Moorhead, P.; Saksela, E. Transformation of cultures of human tissue infected with simian virus 40. J. Cell. Comp. Physiol. 59: 281–292; 1962.

    Article  Google Scholar 

  5. Milo, G. E., Jr.; Di Paolo, J. A. Neoplastic transformation of human diploid cells in vitro after chemical carcinogen treatment. Nature 275: 130–132; 1978.

    Article  PubMed  CAS  Google Scholar 

  6. Reznikoff, C. A.; De Mars, R. In vitro chemical mutagenesis and viral transformation of a human endothelial cell strain. Cancer Res. 41: 1114–1126; 1981.

    PubMed  CAS  Google Scholar 

  7. Shein, H.; Enders, J. Transformation induced by simian virus 40 in human renal cell cultures: I. Morphology and growth characteristics. Proc. Natl. Acad. Sci. USA 48: 1164–1172; 1962.

    Article  PubMed  CAS  Google Scholar 

  8. Todaro, G. J.; Green, H.; Swift, M. R. Susceptibility of human diploid fibroblast strains to transformation by SV40 virus. Science 153: 1252–1254; 1966.

    Article  PubMed  CAS  Google Scholar 

  9. Brooks, S. E.; Amsterdam, D.; Hoffman, L. M.; Adachi, M.; Schneck, L. Cytology, growth characteristics and cellular alterations following SV40-induced transformation of human foetal brain cells derived from aG m2 gangliosidosis and control. J. Cell Sci. 38: 211–223; 1979.

    PubMed  CAS  Google Scholar 

  10. Risser, R.; Pollack, R. A. A nonselective analysis of SV40 transformation of mouse 3T3 cells. Virology 59: 477–489; 1974.

    Article  PubMed  CAS  Google Scholar 

  11. Macpherson, I.; Montagnier, L. Agar suspension culture for the selective assay of cells transformed by Polyoma virus. Virology 23: 291–294; 1964.

    Article  PubMed  CAS  Google Scholar 

  12. Morrhead, P. S.; Nowell, P. C.; Mellman, W. J.; Battips, D. M.; Hungerford, D. A. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp. Cell Res. 20: 613–616; 1960.

    Article  Google Scholar 

  13. Summer, A. T.; Evans, H. S.; Buckland, R. A. A new technique for distinguishing between human chromosomes. Nature 232: 31–39; 1971.

    Google Scholar 

  14. Caspersson, T.; Lomakka, G.; Zeck, L. The 24 fluorescent patterns of the human metaphase chromosomes: Distinguishing characters and variability. Hereditas 67: 89–102; 1971.

    Article  Google Scholar 

  15. ISCN, An international system for human cytogenetic nomenclature. Birth Defects: Original Article Series, New York, The National Foundation, 1978, vol. XIV, No. 8.

    Google Scholar 

  16. Hogan, M. J.; Alvaro, J. A.; Weddell, J. E. Choroid. Histology of the human eye. Philadelphia: W. B. Saunders Co.; 1971: 320–392.

    Google Scholar 

  17. Black, P. H. The oncogenic DNA viruses. A review of in vitro transformation studies. Ann. Rev. Microbiol. 22: 391–426; 1968.

    Article  CAS  Google Scholar 

  18. Aaronson, S. A. Susceptibility of human cell strains to transformation by simian virus 40 and simian virus 40 deoxyribonucleic acid. J. Virol. 6: 470–475; 1970.

    PubMed  CAS  Google Scholar 

  19. Aaronson, S. A.; Todaro, G. J. SV40 T-antigen induction and transformation in human fibroblast cell strais. Virology 36: 254–261; 1968.

    Article  PubMed  CAS  Google Scholar 

  20. Gotoh, S.; Gelb, L.; Schlessinger, D. SV40-transformed human diploid cells that remain transformed throughout their limited lifespan. J. Gen. Virol. 42: 409–414; 1979.

    PubMed  CAS  Google Scholar 

  21. Jensen, F.; Koprowski, H.; Ponten, J. A. Rapid transformation of human fibroblast cultures by simian virus 40. Proc. Natl. Acad. Sci. USA 50: 343–348; 1963.

    Article  PubMed  CAS  Google Scholar 

  22. Potter, C. W.; Potter, A. M.; Oxford, J. S. Comparison of transformation and T-antigen induction in human cell lines. J. Virol. 5: 293–298; 1970.

    PubMed  CAS  Google Scholar 

  23. Santoli, D.; Wroblewska, Z.; Gilden, D. H.; Giraldi, A.; Koprowski, H. Human brain tissue culture. III. PML-SV40-induced transformation of brain cells and establishment of permanent lines. J. Comp. Neurol. 161: 317–328; 1975.

    Article  PubMed  CAS  Google Scholar 

  24. Shein, H. M. Transformation of astrocytes and destruction of spongioblasts by simian tumor virus 40 (SV40) in cultures of human fetal neuroglia. J. Neuropath. Exp. Neurol. 26: 309–326; 1967.

    Article  Google Scholar 

  25. Polani, P. E. DNA repair defects and chromosome instability disorders. Human genetics: Possibilities and realities. Ciba Foundation symposium 66. Amsterdam: Excerpta Medica; 1979; 81–127.

    Google Scholar 

  26. Todaro, G. J.; Martin, G. M. Increased susceptibility of Down’s syndrome fibroblasts to transformation by SV40 virus. Proc. Soc. Exp. Biol. Med. 124: 1232–1236; 1967.

    PubMed  CAS  Google Scholar 

  27. Wolman, S. R.; Steinberg, M. L.; Defendi, V. Simian virus 40-induced chromosome changes in human epidermal cultures. Cancer Genet. Cytogenet. 2: 39–46; 1980.

    Article  Google Scholar 

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Authors and Affiliations

  1. Institute of Microbiology, University of Rome, Rome, Italy

    Giuseppe Carruba

  2. Institute of Human Genetics, University of Urbino, Urbino, Italy

    Bruno Dallapiccola

  3. Institute of Medical Genetics, University of Rome, Rome, Italy

    Viviana Brinchi

  4. C.N.R. Center for Cytopharmacology, University of Milan, via Vanvitelli, 32, 20129, Milano, Italy

    Carlo De Giuli Morghen

Authors
  1. Giuseppe Carruba
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  2. Bruno Dallapiccola
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  3. Viviana Brinchi
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  4. Carlo De Giuli Morghen
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Additional information

This work was supported by Grant 82.00346.96 from the C.N.R. finalized project “Control of Neoplastic Growth”.

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Carruba, G., Dallapiccola, B., Brinchi, V. et al. Ultrastructural and biological characterization of human choroid cell cultures transformed by Simian Virus 40. In Vitro 19, 443–452 (1983). https://doi.org/10.1007/BF02619591

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  • DOI: https://doi.org/10.1007/BF02619591

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