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Methods for production of monoclonal antibodies with specificity for human lung cancer cells

  • Symposium-Tissue Culture Association
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Summary

We have developed a screening strategy and technology to produce monoclonal antibodies with specificity for human lung cancer cells. Mice and rats were immunized with well-characterized tissue culture lines of human small cell lung cancer (SCLC), mouse myeloma x spleen hybrids formed by the technique of Kohler and Milstein, and the resulting culture fluids were screened for antibody binding phenotype using a radioimmunoassay. To facilitate testing large numbers of culture fluids, a 96-well, microtiter based, resuable, replicating device was designed. Using this, many hybridoma culture fluids were replica plated for antibody binding tests on a series of human target cell plates. Hybrids producing antibodies that reacted with the immunizing SCLC line and another independent SCLC line, but not with autologous B-lymphoblastoid cells derived from one of the patients, were identified, selected, and then repeatedly recloned using the same screening strategy. With this technology, hybridomas representing less than 0.5% of all hybrids generated could be isolated and stable antibody producing cultures derived. Such antibodies reacted with a panel of well-characterized SCLC lines and SCLC samples taken directly from patients but not with a variety of normal tissues. Using these antibodies we can demonstrate: tumor cell contamination of bone marrow specimens, marked heterogeneity of antigen expression on cells within individual SCLC lines and individual patients, and inhibition of clonal growth of SCLC lines in soft agarose assays. All of these findings have potential clinical and cell biologic application.

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Authors and Affiliations

  1. National Cancer Institute-Veterans Administration, Washington, D.C.

    John D. Minna, Frank Cuttitta, Steven Rosen, Paul A. Bunn Jr., Desmond N. Carney, Adi F. Gazdar & Stephen Krasnow

  2. National Cancer Institute-Navy Medical Oncology Branch, Division of Cancer Treatment, National Cancer Institute, Washington, D.C.

    John D. Minna, Frank Cuttitta, Steven Rosen, Paul A. Bunn Jr., Desmond N. Carney, Adi F. Gazdar & Stephen Krasnow

  3. Veterans Administration Medical Center, 20814, Bethesda, Maryland

    John D. Minna, Frank Cuttitta, Steven Rosen, Paul A. Bunn Jr., Desmond N. Carney, Adi F. Gazdar & Stephen Krasnow

  4. National Naval Medical Center, 20814, Bethesda, Maryland

    John D. Minna, Frank Cuttitta, Steven Rosen, Paul A. Bunn Jr., Desmond N. Carney, Adi F. Gazdar & Stephen Krasnow

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  1. John D. Minna
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  2. Frank Cuttitta
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  4. Paul A. Bunn Jr.
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Additional information

This symposium was supported in part by the following organizations: Bethesda Research Laboratories, Cetus Corporation, Hybritech Incorporated, MAB-Monoclonal Antibodies, Inc., National Capital Area Branch of the Tissue Culture Association, New England Nuclear Corporation, and Ortho Pharmaceutical Corporation.

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Minna, J.D., Cuttitta, F., Rosen, S. et al. Methods for production of monoclonal antibodies with specificity for human lung cancer cells. In Vitro 17, 1058–1070 (1981). https://doi.org/10.1007/BF02618603

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