Summary
Thin layer chromatography of [14C]palmitate labeled phospholipids of mosquitoAedes aegypti cells reveals that phosphatidylethanolamine is the major phospholipid, and ceramide phosphorylcholine the major sphingolipid. Glycolipids of these cells contain mannose in addition to glucose. The distribution of phospholipids in subcellular membrane fractions shows an enrichment in sphingomyelin and phosphatidyl serine in plasma membranes and other smooth membrane fractions. Cardiolipin is located predominantly in fractions, rich in mitochondrial membranes.
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Townsend, D.; Jenkin, H. M.; Yang, T. K. Lipid analysis ofAedes aegypti cells cultivatedin vitro. Biochim. Biophys. Acta 260: 20–25; 1972.
Luukkonen, A.; Brummer-Korvenkontio, M.; Renkonen, O. Lipids of cultured mosquito cells (Aedes albopictus). Comparison with cultured mammalian fibroblasts (BHK 21 cells). Biochim. Biophys. Acta 326: 256–261; 1973.
Masoro, E. J. Lipids and lipid metabolism. Ann. Rev. Physiol. 39: 301–321; 1977.
Varma, M. R. G.; Pudney, M. The growth and serial passage of cell lines fromAedes aegypti (L.) larvae in different media. J. Med. Entomol. 6: 432–439; 1969.
Butters, T. D.; Hughes, R. C. Solubilization and fractionation of glycoproteins and glycolipids of KB cell membranes. Biochem. J. 140: 469–478; 1974.
Gray, G. M. Chromatography of lipids. I. An improved chromatographic procedure for the quantitative isolation of the neutral ceramidecontaining glycolipids from mammalian cells. Biochim. Biophys. Acta 144: 511–518; 1967.
Bray, G. A. A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Anal. Biochem. 1: 279–285; 1960.
Dittmer, J. C.; Lester, R. L. A simple specific spray for the detection of phospholipids on thinlayer chromatograms. J. Lipid Res. 5: 126–127; 1964.
Owens, K. A two-dimensional thin-layer chromatographic procedure for the estimation of plasmalogens. Biochem. J. 100: 354–361; 1966.
Norton, W. T. Reaction of mercuric chloride with plasmalogen. Nature 184: 1144–1145; 1959.
Clamp, J. R. Analysis of glycoproteins. Biochem. Soc. Symp. 40: 3–16; 1974.
Fast, P. G. A comparative study of the phospholipids and fatty acids of some insects. Lipids 1: 209–215; 1966.
Dawson, R. M. C.; Kemp, P. Isolation of ceramide phosphorylethanolamine from the blowflyCalliphora erythrocephala. Biochem. J. 106: 319–320; 1968.
Sugita, M.; Shirai, S.; Itasaka, O.; Hori, T. Neutral glycosphingolipids containing mannose from the bivalveCorbicula sandai. J. Biochem. 77: 125–130; 1975.
Butters, T. D.; Hughes, R. C. Lectin binding to mosquitoAedes aegypti and human KB cells: structural comparisons of membrane oligosaccharides. Carbohydr. Res. 61: 159–168; 1978.
Zambrano, F.; Fleischer, S.; Fleischer, B. Lipid composition of the Golgi apparatus of rat kidney and liver in comparison with other subcellular organelles. Biochim. Biophys. Acta 380: 357–369; 1975.
Brotherus, J.; Renkonen, O. Phospholipids of subcellular organelles isolated from cultured BHK cells. Biochim. Biophys. Acta 486: 243–253; 1977.
Micklem, K. J.; Abra, R. M.; Knutton, S.; Graham, J. M.; Pasternak, C. A. The fluidity of normal and virus-transformed cell plasma membrane. Biochem. J. 154: 561–566; 1976.
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Butters, T.D., Hughes, R.C. Phospholipids and glycolipids in subcellular fractions of mosquitoAedes aegypti cells. In Vitro 17, 831–838 (1981). https://doi.org/10.1007/BF02618451
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DOI: https://doi.org/10.1007/BF02618451