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In vitro culture of tobacco protoplasts: Use of feeder techniques to support division of cells plated at low densities

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Summary

Tobacco protoplasts obtained from leaf mesophyll cells and suspended in agar nutrient medium divided and produced colonies only when plated at high densities, above 104 cells per ml. At such densities coalescence of the expanding colonies occurred at high frequency.

Nondividing X-irradiated protoplasts used as feeder cells supported division of viable protoplasts plated at densities as low as 102 cells per ml. The feeder cell technique should thus facilitate the application of screening procedures for the isolation of colonies originating from single mutated cells occurring in a suspended population.

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Author notes

  1. Eliezer Huberman

    Present address: Department of Genetics, The Weizmann Institute of Science, Rehovot, Israel

Authors and Affiliations

  1. Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel

    Dina Raveh, Eliezer Huberman & Esra Galun

Authors
  1. Dina Raveh
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  2. Eliezer Huberman
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  3. Esra Galun
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Raveh, D., Huberman, E. & Galun, E. In vitro culture of tobacco protoplasts: Use of feeder techniques to support division of cells plated at low densities. In Vitro 9, 216–222 (1973). https://doi.org/10.1007/BF02618439

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  • DOI: https://doi.org/10.1007/BF02618439

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