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Primary cultures of fetal hepatocytes from the genetically obese Zucker rat: Characterization and total lipogenesis

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Summary

Primary fetal hepatocyte cultures derived from Zucker rats and with expectedfa-gene frequencies of 0.0 and 0.75 have been established and can be used to detect early effects of thefa gene on hepatocellular metabolism. Proliferative capacity is similar in both types of culture. Changes of the growth media significantly decrease total lipogenesis in both 0.0 and 0.75fa-gene culture grown in arginine-free DME medium. Paired incubation experiments demonstrate that total lipogenesis in 0.75fa gene cultures is significantly less than in 0.0fa-gene cultures under basal conditions. Stimulation of total lipogenesis by pharmacological doses of insulin and excess substrate (glucose) is significantly less in the 0.75fa gene than in the 0.0fa-gene cultures. These data suggest that the development of obesity in the Zucker rat cannot be attributed to elevated hepatic lipogenesis in the fetus.

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Authors and Affiliations

  1. Department of Biology, Vassar College, 12601, Poughkeepsie, New York

    A. L. Goldstein & P. R. Johnson

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  1. A. L. Goldstein
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  2. P. R. Johnson
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Additional information

This work constitutes part of a Ph.D. dissertation submitted to New York University by A. L. Goldstein. A portion of this work was presented in preliminary form at the 1978 meeting of the Tissue Culture Association.

Supported in part by National Institutes of Health Grant AM 19382 and a Grant from the Weight Watchers Foundation, Inc.

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Goldstein, A.L., Johnson, P.R. Primary cultures of fetal hepatocytes from the genetically obese Zucker rat: Characterization and total lipogenesis. In Vitro 16, 288–296 (1980). https://doi.org/10.1007/BF02618334

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  • DOI: https://doi.org/10.1007/BF02618334

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