Summary
Eighteen lots of fetal bovine serum were tested for their ability to support clonal growth and 3-methylcholanthrene-induced morphological transformation of hamster embryo cells in vitro. Most of them supported cloning efficiencies of over 11%. However, cloning efficiency alone was an inadequate criterion for selecting serum for transformation studies, since no transformation was observed with some lots, even though their cloning efficiencies were over 16%. This shows the importance of pretesting serum for its ability to support morphological transformation before it is used in mammalian cell carcinogenesis tests.
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Research sponsored by the National Cancer Institute under Contract No. N01-CO-75380 with Litton Bionetics, Inc.
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Schuman, R.F., Pienta, R.J., Poiley, J.A. et al. Effect of fetal bovine serum on 3-methylcholanthrene-induced transformation of hamster cells in vitro. In Vitro 15, 730–735 (1979). https://doi.org/10.1007/BF02618253
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DOI: https://doi.org/10.1007/BF02618253