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Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture

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Summary

A new human endometrial cell line, RL95-2, derived from a Grade 2 moderately differentiated adenosquamous carcinoma of the endometrium has been passaged successfully in cell culture for more than 2 yr. The cells are characteristically epithelioid with well-defined junctional complexes, tonofilaments, filopodialike extensions, and surface microvilli. Nuclei are large, irregular, and invaginated frequently with multiple, prominent, lamellar nucleoli. The cells have a log phase doubling time of 22 to 34 h followed by continued growth at a reduced rate with no apparent plateau phase. They exhibit a strong tendency for piling up as well as for the formation of glandlike dome structures. Karyotypically the line is trisomic 8 (47,XX,+8) and has an 8% frequency of polyploidization. Both cytoplasmic and nuclear estrogen receptors are present. Antihuman α-keratin characterizes the cell line as epithelial, nonstromal. The RL95-2 cell line may provide a useful in vitro system for the investigation of the endocrine regulation of endometrial neoplasia.

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Authors and Affiliations

  1. Department of Pathology University of Arizona, College of Medicine, 85724, Tucson, Arizona

    Dennis L. Way, David S. Grosso, John R. Davis, Earl A. Surwit & C. D. Christian

  2. Department of Obstetrics and Gynecology University of Arizona, College of Medicine, 85724, Tucson, Arizona

    Dennis L. Way, David S. Grosso, John R. Davis, Earl A. Surwit & C. D. Christian

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  1. Dennis L. Way
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  2. David S. Grosso
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Way, D.L., Grosso, D.S., Davis, J.R. et al. Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture. In Vitro 19, 147–158 (1983). https://doi.org/10.1007/BF02618053

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