Summary
Two cell lines derived from primary human renal-cell cancers (RCC) have been established and characterized. Cell line 786-O has been in culture for longer than 1 year and has been subcultured more than 50 times. It has a doubling time of 45 hr and a hypertriploid karyotype and possesses a Y chromosome. Cell line 769-P also has been in culture for longer than 1 year. It has been subcultured 50 times and has a doubling time of 35 hr and a hypodiploid karyotype. Cells from both lines are epithelial, and they produce tumors in the cheek pouches of immunosuppressed hamsters. Neither cell line is contaminated withMycoplasma. Cells of the two lines can be distinguished from HeLa cells both by their karyotypes and by the mobility patterns of their isoenzymes of glucose-6-phosphate dehydrogenase.
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Williams, R. D., A. Y. Elliott, N. Stein, and E. E. Fraley. 1976. In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 323–327.
Fogh, J., and G. Trempe. 1975. New human tumor cell lines. In: J. Fogh (Ed.),Human Tumor Cells in Vitro. Plenum Press, New York, pp. 115–160.
Elliott, A. Y., D. L. Bronson, N. Stein, and E. E. Fraley. 1976. In vitro cultivation of epithelial cells derived from tumors of the human urinary tract. Cancer Res. 36: 365–369.
Liao, S. K., P. B. Dent, and P. B. McCulloch. 1975. Characterization of human malignant melanoma cell lines. I. Morphology and growth characteristics in culture. J. Nat. Cancer Inst. 54: 1037–1044.
Elliott, A. Y., P. Cleveland, J. Cervenka, A. E. Castro, N. Stein, T. R. Hakala, and E. E. Fraley. 1974. Characterization of a cell line from human transitional cell cancer of the urinary tract. J. Nat. Cancer Inst. 55: 1341–1394.
Caspersson, T., L. Zech, and C. Johansson. 1970. Analysis of the human metaphase chromosome set by aid of DNA binding fluorescent agents. Exp. Cell Res. 62: 490–492.
Kirkman, H. N. 1961. Starch gel electrophoresis of human glucose-6-phosphate-dehydrogenase. South. Med. J. 54: 1431–1432.
Nordquist, R. E., D. R. Ishmael, C. A. Lovig, D. M. Hyder, and A. F. Hoge. 1975. The tissue culture and morphology of human breast tumor cell line BOT-2. Cancer Res. 35: 3100–3105.
Elhilali, M. M., S. K. Nayak, and C. Brailovsky. 1972. A transplantable human bladder cancer in unconditioned Syrian hamster. Invest. Urol., 10: 230–234.
Lewis, L. J., J. C. Hoak, R. D. Maca, and G. L. Fry. 1973. Replication of human endothelial cells in culture. Science 181: 453–454.
Nelson-Rees, W. A., and R. R. Flandermeyer. 1976. HeLa cultures defined. Science 191: 96–98.
Owens, R. B., H. S. Smith, W. A. Nelson-Rees, and E. L. Springer. 1976. Epithelial cell cultures from normal and cancerous human tissues. J. Nat. Cancer. Inst. 56: 843–849.
Tannebaum, M. 1971. Ultrastructural pathology of human renal cell tumors. Pathol. Ann. 6: 249–277.
Gonzalez, R., M. Dempsey, and E. E. Fraley. 1977. Sterol synthesis in human renal cell cancer. J. Urol. 117: 708–711.
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American Cancer Society Clinical Faculty Fellow, 1977–1980.
Supported in part by Grants CA 13095-03 and CA 15551-03 from the National Cancer Institute.
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Williams, R.D., Elliott, A.Y., Stein, N. et al. In vitro cultivation of human renal cell cancer. In Vitro 14, 779–786 (1978). https://doi.org/10.1007/BF02617972
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DOI: https://doi.org/10.1007/BF02617972