Summary
The number of epidermal melanocytes positive to the dopa reaction increased when skin explants from newborn mice were cultured with MSH or dbc-AMP. These agents seem to induce melanogenesis in the pre-existing melanoblasts. This hormone-induced melanogenesis is suppressed by actinomycin D or cycloheximide, suggesting that the initiation of melanogenesis in the epidermal melanoblasts requires de novo transcription and translation.
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References
Rawles, M. E. 1940. The development of melanophores from embryonic mouse tissues grown in the coelom of chick embryo. Proc. Natl. Acad. Sci. U.S.A. 26: 673–680.
Rawles, M. E. 1947. Origin of pigment cells from the neural crest in the mouse embryo. Physiol. Zool. 20: 248–265.
Rawles, M. E. 1948. Origin of melanophores and their role in development of color patterns in vertebrates. Physiol. Rev. 28: 383–408.
Reynolds, J. 1954. The epidermal melanocytes of mice. J. Anat. 88: 45–58.
Rovee, D. T., and W. M. Reams 1964. An experimental and descriptive analysis of the melanocyte population in the venter of the PET mouse. Anat. Rec. 149: 181–190.
Takeuchi, T. 1968. Genetic analysis of a factor regulating melanogenesis in the mouse melanocyte. Jap. J. Genet. 43: 249–256.
Hirobe, T., and T. Takeuchi 1977. Induction of melanogenesis in the epidermal melanoblasts of newborn mice by MSH. J Embryol. Exp. Morphol. 37: 79–90.
Wong, G., and J. Pawelek 1973. Control of phenotypic expression of cultured melanoma cells by melanocyte stimulating hormones. Nature, [New Biol.] 241: 213–215.
Pawelek, J., M. Sansone, J. Morowitz, G. Moellmann, and E. Godawska. 1974. Genetic control of melanization: isolation and analysis of amelanotic variants from cultured melanotic melanoma cells. Proc. Natl. Acad. Sci. U.S.A. 71: 1073–1077.
Wong, G., J. Pawelek, M. Sansone, and J. Morowitz. 1974. Response of mouse melanoma cells to melanocyte stimulating hormone. Nature 248: 351–354.
Kitano, Y. 1974. Response of melanocytes to cyclic AMP. Jap. J. Dematol. 84: 598–603.
Johnson, G. S., and I. Pastan 1972. N6, O2′-dibutyryl adenosine 3′,5′-monophosphate induces pigment production in melanoma cells. Nature [New Biol.] 237: 267–268.
Kreider, J. W., M. Rosenthal, and N. Lengle. 1973. Cyclic adenosine 3′,5′-monophosphate in the control of melanoma cell replication and differentiation. J. Natl. Cancer Inst. 50: 555–558.
Kitano, Y. 1973. Stimulation of dendritogenesis in human melanocytes by dibutyryl adenosine 3′,5′-cyclic monophosphatein vitro. Arch. Dermatol. Forsch. 248: 145–148.
Kuo, J. F., and P. Greengard 1969. Cyclic nucleotide-dependent protein kinase. IV. Widespread occurrence of adenosine 3′,5′-monophosphate-dependent protein kinase in various tissues and phyla of the animal kingdom. Proc. Natl. Acad. Sci. U.S.A. 69: 1349–1355.
Kreider, J. W., D. R., Wade, M. Rosenthal, and T. Densley. 1975. Maturation and differentiation of B-16 melanoma cells induced by theophylline treatment. J. Natl. Cancer Inst. 54: 1457–1467.
Lincoln, T. M., and G. L. Vaughan. 1975. The role of adenosine 3′,5′-monophosphate in the transformation of Cloudman mouse melanoma cells. J. Cell. Physiol. 86: 543–552.
Steinberg, M. L., and J. R. Whittaker. 1976. Stimulation of melanotic expression in a melanoma cell line by theophylline. J. Cell. Physiol. 87: 265–276.
Pawelek, J., M. Sansone, N. Koch, G. Chris, R. Halaban, J. Handee, A. B. Lerner, and J. M. Varga. 1975. Melanoma cells resistant to inhibition of growth by melanocyte stimulating hormone. Proc. Natl. Acad. Sci. U.S.A. 72: 951–955.
Wong, G., and J. Pawelek. 1975. Melanocyte stimulating hormone promotes activation of preexisting tyrosinase molecules in Cloudman S 91 melanoma cells. Nature 255: 644–646.
Hall, T. C., B. H. McCracken, and G. W. Thorn. 1953. Skin pigmentation in relation to adrenal cortical function. J. Clin. Endocrinol. Metab. 13: 243–257.
Bischitz, P. G., and R. S. Snell. 1960. A study of the effect of ovariectomy, oestrogen and progesterone on the melanocytes and melanin in the skin of the female guinea pig. J. Endocrinol. 20: 312–319.
Snell, R. S., and P. G. Bischitz. 1960. The effect of large doses of estrogen and estrogen and progesterone on melanin pigmentation. J. Invest. Dermatol. 35: 73–82.
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This work was supported in part by Grant 944008 from the Ministry of Education
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Hirobe, T., Takeuchi, T. Induction of melanogenesis in vitro in the epidermal melanoblasts of newborn mouse skin by MSH. In Vitro 13, 311–315 (1977). https://doi.org/10.1007/BF02616177
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DOI: https://doi.org/10.1007/BF02616177