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Some aspects of somatic cell hybridization

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References

  1. Krooth, R. S., G. A. Darlington, and A. A. Velasquez. 1968. The genetics of cultured mammalian cells. Ann. Rev. Genet. 2: 141–164.

    Article  Google Scholar 

  2. Ephrussi, B., and M. C. Weiss. 1969. Hybrid somatic cells. Sci. Amer. 220: 26–35.

    PubMed  CAS  Google Scholar 

  3. Flaxman, B. A., M. A. Lutzner, and E. J. Van Scott. 1967. Cell maturation and tissue organization in epithelial outgrowths from skin and buccal mucosain vitro. J. Invest. Derm. 49: 322–332.

    Article  Google Scholar 

  4. Okada, Y. 1962. Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. Exp. Cell Res. 26: 98–107.

    Article  PubMed  CAS  Google Scholar 

  5. Harris, H., and J. F. Watkins. 1965. Hybrid cells derived from mouse and man: artificial heterokaryons of mammalian cells from different sepcies. Nature (London) 205: 640–646.

    Article  CAS  Google Scholar 

  6. Ephrussi, B., and M. C. Weiss. 1967. Regulation of the cell cycle in mammalian cells: inferences and speculations based on observations of interspecific somatic hybrids. Develop. Biol., suppl. 1, 136–139.

    Google Scholar 

  7. Barski, G., S. Sorieul, and F. Cornefert. 1961. “Hybrid” type cells in combined cultures of two different mammalian cell strains. J. Nat. Cancer Inst. 26: 1269–1291.

    PubMed  CAS  Google Scholar 

  8. Lederberg, J. 1958. Genetic approaches to somatic cell variation: summary comment. J. Cell. Comp. Physiol., suppl, 1, 383–392.

    Article  Google Scholar 

  9. Szybalski, W., E. Szybalska, and G. Ragni. 1962. Genetic studies with human cell lines. Nat. Cancer Inst. Monogr. 7: 75–89.

    Google Scholar 

  10. Kit, S., D. R. Dubbs, L. J. Piekarski, and T. C. Hsu. 1963. Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31: 297–312.

    Article  PubMed  CAS  Google Scholar 

  11. Littlefield, J. W. 1964. Three degrees of guanylic acid-inosinic acid pyrophosphorylase deficiency in mouse fibroblasts. Nature (London) 203: 1142–1144.

    Article  CAS  Google Scholar 

  12. Littlefield, J. W. 1965. Studies on thymidine kinase in cultured mouse fibroblasts. Biochim. Biophys. Acta 95: 14–22.

    PubMed  CAS  Google Scholar 

  13. Littlefield, J. W. 1964. Selection of hybrids from matings of fibroblastin vitro and their presumed recombinants. Science 145: 709–710.

    Article  PubMed  CAS  Google Scholar 

  14. Littlefield, J. W. 1966. The use of drug-resistant markers to study the hybridization of mouse fibroblasts. Exp. Cell Res. 41: 190–196.

    Article  PubMed  CAS  Google Scholar 

  15. Weiss, M. C., and H. Green. 1967. Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proc. Nat. Acad. Sci. U. S. A. 58: 1104–1111.

    Article  CAS  Google Scholar 

  16. Weiss, M. C., B. Ephrussi, and L. J. Scaletta. 1968. Loss of T-antigens from somatic hybrids between mouse cells and SV40-transformed human cells. Proc. Nat. Acad. Sci. U. S. A. 59: 1132–1135.

    Article  CAS  Google Scholar 

  17. Marin, G., and J. W. Littlefield. 1968. Selection of morphologically normal cell lines from polyoma-transformed BHK21/13 hamster fibroblasts. J. Virol. 2: 69–77.

    PubMed  CAS  Google Scholar 

  18. Marin, G. Selection of chromosome segregants in a “hybrid” line of Syrian hamster fibroblasts. Exp. Cell Res., in press.

  19. Davidson, R. L., and B. Ephrussi. 1965. A selective system for the isolation of hybrids between L cells and normal cells. Nature (London) 205: 1170–1171.

    Article  Google Scholar 

  20. Nabholz, M., V. Miggiano, and W. Bodmer. Genetic analysis using human-mouse somatic hybrids. Nature (London), in press.

  21. Scaletta, L. J., and B. Ephrussi. 1965. Hybridization of normal and neoplastic cellsin vitro. Nature (London) 205: 1169–1170.

    Article  Google Scholar 

  22. Weiss, M. C., and B. Ephrussi. Studies of interspecific (rat × mouse) somatic hybrids. II. Lactate dehydrogenase and β-glucuronidase. Genetics 54: 1111–1122.

  23. Green, H., B. Ephrussi, M. Yoshida, and D. Hamerman. 1966. Synthesis of collagen and hyaluronic acid by fibroblast hybrids. Proc. Nat. Acad. Sci. U. S. A. 55: 41–44.

    Article  CAS  Google Scholar 

  24. Spencer, R. A., J. S. Hauschka, D. P. Amos, and B. Ephrussi. 1964. Co-dominance of isoantigens in somatic hybrids of murine cells grownin vitro. J. Nat. Cancer Inst. 33: 893–903.

    PubMed  CAS  Google Scholar 

  25. Davidson, R., and K. Yamamoto. 1968. Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization. II. The level of regulation of 3,4-dihydroxyphenylalanine oxidase. Proc. Nat. Acad. Sci. U. S. A. 60: 894–901.

    Article  CAS  Google Scholar 

  26. Littlefield, J. W. The evidence for bacterial-type regulation in animal cells. In: Twentythird symposium on fundamental cancer research, at the University of Texas-M. D. Anderson Hospital and Tumor Institute, in press.

  27. Roscoe, D. Personal communication.

  28. Koprowski, H., F. C. Jensen, and Z. Steplewski. 1967. Activation of production of infectious tumor virus SV40 in heterokaryon cultures. Proc. Nat. Acad. Sci. U. S. A. 58: 127–133.

    Article  CAS  Google Scholar 

  29. Watkins, J. F., and R. Dulbecco. 1967. Production of SV40 virus in heterokaryons of transformed and susceptible cells. Proc. Nat. Acad. Sci. U. S. A. 58: 1396–1403.

    Article  CAS  Google Scholar 

  30. Kao, F.-T., and T. T. Puck. 1968. Genetics of somatic mammalian cells. VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Nat. Acad. Sci. U. S. A. 60: 1275–1281.

    Article  CAS  Google Scholar 

  31. Chu, E. H. Y., and H. V. Malling. 1968. Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cellsin vitro. Proc. Nat. Acad. Sci. U. S. A. 61: 1306–1312.

    Article  CAS  Google Scholar 

  32. Siniscalco, M., H. P. Klinger, H. Eagle, H. Koprowski, W. Y. Fugimoto, and J. E. Seegmiller. 1969. Evidence for intergenic complementation in hybrid cells derived from two human diploid strains earch carrying an X-linked mutation. Proc. Nat. Acad. Sci. U. S. A. 62: 793–799.

    Article  CAS  Google Scholar 

  33. Littlefield, J. W. 1969. Hybridization of hamster cells with high and low folate reductase activity. Proc. Nat. Acad. Sci. U. S. A. 62: 88–95.

    Article  CAS  Google Scholar 

  34. Seegmiller, J. E., F. M. Rosenbloom, and W. M. Kelley. 1967. Enzyme defect associated with sex-linked human neurological disorder and excessive purine synthesis. Science 155: 1682–1684.

    Article  PubMed  CAS  Google Scholar 

  35. Kakimoto, Y., K. Tanguchi, and I. Sano. 1969.d-\-Aminoisobutyrate: pyruvate aminotransferase in mammalian liver and excretion of β-aminoisobutyrate by man. J. Biol. Chem. 244: 335–340.

    PubMed  CAS  Google Scholar 

  36. Goldsby, R., and R. Breslow. Isolation and characterization of thymidine transport mutants in Chinese hamster cells. Exp. Cell. Res., in press.

  37. Goldstein, S., and J. W. Littlefield. 1968. Selection for or against human fibroblasts lacking hypoxanthine phosphoribosyltransferase. Abstract, Tissue Culture Association, San Juan, June, p. 54.

    Google Scholar 

  38. Friedmann, T., J. E. Seegmiller, and J. H. Subak-Sharpe. 1968. Metabolic cooperation between genetically marked human fibroblasts in tissue culture. Nature (London) 220: 272–274.

    Article  CAS  Google Scholar 

  39. Littlefield, J. W., and C. Basilico. 1966. Infection of thymidine kinase-deficient BHK cells with polyoma virus. Nature (London) 211: 250–252.

    Article  CAS  Google Scholar 

  40. DeMars, R. 1968. The inhibition by glutamine of glutamyl transferase formation in cultures of human cells. Biochim. Biophys. Acta 27: 435–436.

    Article  Google Scholar 

  41. Pollack, R. E., H. Green, and G. J. Todaro. 1968. Growth control in cultured cells: selection of sublines with increased sensitivity to contact inhibition and decreased tumor-producing ability. Proc. Nat. Acad. Sci. U. S. A. 60: 126–133.

    Article  CAS  Google Scholar 

  42. Kao, F.-T., R. T. Johnson, and T. T. Puck. 1969. Complementation analysis on virusfused Chinese hamster cells with nutritional markers. Science 164: 312–314.

    Article  PubMed  CAS  Google Scholar 

  43. Tedesco, T. A., and W. J. Mellman. 1967. Argininosuccinate synthetase activity and citrulline metabolism in cells cultured from a citrullinemic subject. Proc. Nat. Acad. Sci. U. S. A. 57: 829–834.

    Article  CAS  Google Scholar 

  44. Shih, V. E., J. W. Littlefield, and H. W. Moser. 1969. Argininosuccinase deficiency in fibroblasts cultured from patients with argininosuccinic aciduria. Biochem. Genet. 3: 81–83.

    Article  Google Scholar 

  45. Migeon, B. R., and C. S. Miller. 1968. Humanmouse somatic cell hybrids with single human chromosome (group E): link with thymidine kinase activity. Science 162: 1005–1006.

    Article  PubMed  CAS  Google Scholar 

  46. Pontecorvo, G. 1962. Methods of microbial genetics in an approach to human genetics. Brit. Med. Bull. 18: 81–84.

    PubMed  CAS  Google Scholar 

  47. Mazia, D., P. J. Harris, and T. Bibring. 1960. The multiplicity of the mitotic centers and the time-course of their duplication and separation. J. Biophys. Biochem. Cytol. 7: 1–20.

    Article  CAS  PubMed  Google Scholar 

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Author notes

  1. Samuel Goldstein

    Present address: Department of Medicine, Mc-Master University, Hamilton, Ontario, Canada

Authors and Affiliations

  1. Genetics Unit, Children's Service, Massachusetts General Hospital, Boston, Massachusetts

    John W. Littlefield & Samuel Goldstein

  2. Department of Pediatrics, Harvard Medical School, Boston, Massachusetts

    John W. Littlefield & Samuel Goldstein

Authors
  1. John W. Littlefield
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  2. Samuel Goldstein
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Additional information

The studies mentioned were supported by United States Public Health Service Grant CA-04670.

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Littlefield, J.W., Goldstein, S. Some aspects of somatic cell hybridization. In Vitro 6, 21–31 (1970). https://doi.org/10.1007/BF02616131

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