Skip to main content
SpringerLink
Log in

A transitional cell carcinoma cell line

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

A transitional cell carcinoma cell line, COLO 232, was derived from a primary urinary bladder tumor in a Caucasian male. In culture, COLO 232 retained distinct uroepithelial phenotypic traits and produced both carcinoembryonic antigen and adrenocorticotropic hormone. COLO 232 had a chromosome mode of 58 and retained the X and Y chromosomes. Ten marker chromosomes were identified. COLO 232 will be of value for biochemical and immunological studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Moore, G. E., R. E. Gener, and H. A. Franklin. 1967. Culture of normal human leukocytes. J. Am. Med. Assoc. 199: 519–524.

    Article  CAS  Google Scholar 

  2. Woods, L. K., G. E. Moore, C. J. Bainbridge, C. C. Huang, C. Huzella, and L. A. Quinn. 1973. Lymphoid cell lines established from peripheral blood of persons with Down's syndrome. N. Y. State J. Med. 73: 869–873.

    PubMed  CAS  Google Scholar 

  3. Ham, R. G. 1965. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Nat. Acad. Sci. U.S.A. 53: 288–293.

    Article  CAS  Google Scholar 

  4. Moore, G. E., S. B. Merrick, L. K. Woods, and N. M. Arabasz. 1975. A human squamous cell carcinoma cell line. Cancer Res. 35: 2684–2688.

    PubMed  CAS  Google Scholar 

  5. Lubs, H. A., W. H. McKenzie, S. R. Patil, and S. Merrick. 1973. New staining methods for chromosomes. In: D. M. Prescott (Ed.),Methods in Cell Biology. Vol. 6. pp. 346–380.

  6. Seabright, M. 1971. A rapid banding technique for human chromosomes. Lancet 2: 971–972.

    Article  PubMed  CAS  Google Scholar 

  7. McKenzie, W. H., and H. A. Lubs. 1973. An analysis of technical variables in the production of C bands. Chromosoma 41: 175–182.

    Article  PubMed  CAS  Google Scholar 

  8. Krutzik, S. 1976.Radioimmunoassay Manual. 3rd Edition. Nichols Institute, San Pedro, Calif., pp. 209–220.

    Google Scholar 

  9. Eipper, B. A., and R. E. Mains. 1975. High molecular weight forms of adrenocorticotropic hormone in the mouse pituitary and in a mouse tumor cell line. Biochemistry 14: 3836–3844.

    Article  PubMed  CAS  Google Scholar 

  10. Chen, T. R. 1975. Microscopic demonstration of mycoplasma contamination in cell cultures and cell culture media. Procedure 75361. TCA Manual 1: 229–232.

    Article  Google Scholar 

  11. Mizrahi, A., and G. E. Moore. 1971. Long-term preservation of permanent human hematopoietic cell lines. J. Med. 2: 380–386.

    Article  PubMed  CAS  Google Scholar 

  12. Firth, J. A., and R. M. Hicks. 1973. Interspecies variation in the fine structure and enzyme cytochemistry of mammalian transitional epithelium. J. Anat. 116: 31–43.

    PubMed  CAS  Google Scholar 

  13. Quinn, L. A., L. K. Woods, S. B. Merrick, N. M. Arabasz, and G. E. Moore. 1977. Cytogenetic analysis of 12 human malignant melanoma cell lines. J. Nat. Cancer Inst. 59: 301–307.

    Google Scholar 

  14. O'Toole, C., S. Mayak, Z. Price, W. H. Gilbert, and J. Waisman. 1976. A cell line (SCaBER) derived from squamous cell carcinoma of the human urinary bladder. Int. J. Cancer 17: 707–714.

    Article  PubMed  Google Scholar 

  15. Elliot, A. Y., P. Cleveland, J. Cervenka, A. E. Castro, N. Stein, T. R. Hakala, and E. E. Fraley. 1974. Characterization of a cell line from human transitional cell carcinoma of the urinary tract. J. Nat. Cancer Inst. 53: 1341–1349.

    Google Scholar 

  16. Rigby, C. C., and L. M. Franks. 1970. A human tissue culture cell line from a transitional cell tumor of the urinary bladder: Growth, chromosome pattern and ultrastructure. Br. J. Cancer 24: 746–754.

    PubMed  CAS  Google Scholar 

  17. Bubenik, J., M. Baresova, V. Viklicky, J. Jakoubkova, H. Sainerova, and J. Donner. 1973. Established cell line of urinary bladder carcinoma (T24) containing tumor-specific antigen. Int. J. Cancer 11: 765–773.

    Article  PubMed  CAS  Google Scholar 

  18. Rasheed, S., M. B. Gardner, R. W. Rongey, W. A. Nelson-Rees, and P. Arnstein. 1977. Human bladder carcinoma: Characterization of two new tumor cell lines and search for tumor viruses. J. Nat. Cancer Inst. 58: 881–890.

    PubMed  CAS  Google Scholar 

  19. Liddle, G. W., W. E. Nicholson, D. P. Island, D. N. Orth, K. Abe, and S. C. Lowder. 1969. Clinical and laboratory studies of ectopic hormonal syndromes. Rec. Prog. Horm. Res. 25: 283–293.

    PubMed  CAS  Google Scholar 

  20. Morgan, R. T., L. A. Quinn, L. K. Woods, and G. E. Moore. 1977. Marker properties of tumor and lymphoid cell lines derived from a patient with squamous cell carcinoma. Cancer Res. 37: 2030–2035.

    PubMed  CAS  Google Scholar 

  21. Gerwirtz, G., and R. S. Yalow. 1974. Ectopic ACTH production in carcinoma of the lung. J. Clin. Invest. 53: 1022–1032.

    Article  Google Scholar 

  22. Leibovitz, A., J. C. Stinson, W. B. McCombs, III, C. E. McCoy, K. C. Mazur, and N. D. Mabry. 1976. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562–4569.

    PubMed  CAS  Google Scholar 

  23. Drewinko, B., M. M. Romsdahl, L. Y. Yang, M. J. Ahearn, and J. M. Trujillo. 1976. Establishment of a human carcinoembryonic antigenproducing colon adenocarcinoma cell line. Cancer Res. 36: 467–475.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Denver General Hospital, W. 8th Avenue and Cherokee Street, 80204, Denver, Colorado

    George E. Moore, Robert T. Morgan, Laurie A. Quinn & Linda K. Woods

Authors
  1. George E. Moore
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert T. Morgan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Laurie A. Quinn
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Linda K. Woods
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by Grant No. CA 15018 awarded by the National Cancer Institute, DHEW, and the Mary B. and L. H. Marshall Fund.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moore, G.E., Morgan, R.T., Quinn, L.A. et al. A transitional cell carcinoma cell line. In Vitro 14, 301–306 (1978). https://doi.org/10.1007/BF02616040

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02616040

Key words

Search

Navigation